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Nucleation and Crystal Growth of Hemoglobins

The Case of HbC

  • Protocol
Hemoglobin Disorders

Part of the book series: Methods in Molecular Biology™ ((MIMM,volume 82))

Abstract

Hemoglobin (Hb) crystallization is of significance both in vivo and in vitro. Hb crystals form in red blood cells (RBCs), as occurs in the case of patients expressing βC-globin (β6 Glu→Lys). In vitro, high-resolution structural determination by crystallographic methods requires the growth of Hb crystals to approx ∼1 mm in diameter, which may be induced by a variety of precipitants.

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Author information

Authors and Affiliations

  1. Department of Chemical Engineering, University of Houston, Houston, TX

    Peter G. Vekilov

  2. Sue Golding Graduate Division, Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY

    Angela Feeling-Taylor

  3. Medical Scientists Training Program, Albert Einstein College of Medicine, Bronx, NY

    Angela Feeling-Taylor

  4. Division of Hematology, Department of Medicine, Albert Einstein College of Medicine, Bronx, NY

    Rhoda Elison Hirsch

  5. Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY

    Rhoda Elison Hirsch

Authors
  1. Peter G. Vekilov
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  2. Angela Feeling-Taylor
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  3. Rhoda Elison Hirsch
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Editor information

Editors and Affiliations

  1. Albert Einstein College of Medicine, Bronx, NY

    Ronald L. Nagel MD

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© 2003 Humana Press Inc.

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Vekilov, P.G., Feeling-Taylor, A., Hirsch, R.E. (2003). Nucleation and Crystal Growth of Hemoglobins. In: Nagel, R.L. (eds) Hemoglobin Disorders. Methods in Molecular Biology™, vol 82. Humana Press. https://doi.org/10.1385/1-59259-373-9:155

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  • DOI: https://doi.org/10.1385/1-59259-373-9:155

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-962-9

  • Online ISBN: 978-1-59259-373-6

  • eBook Packages: Springer Protocols

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