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Elucidation of Signal Transduction Pathways by Transfection of Cells with Modified Oncogenes

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Cancer Cell Signaling

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 218))

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Abstract

This chapter will focus on introduction of various wild type (WT) and mutant genes into cells by DNA transfection. Techniques for analysis of the inheritance, expression, and biological effects of the introduced genes will be described. Various strong and weak points about three different techniques of stable gene transfer, including calcium-phosphate DNA precipitation, transfection via liposomes, and transfection via electroporation, will be discussed.

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Author information

Authors and Affiliations

  1. Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Greenville, NC

    Linda S. Steelman, Phillip W. Moye, John T. Lee, John G. Shelton, Patrick M. Navolanic, Steven L. Knapp, Richard A. Franklin & James A. McCubrey

  2. Shands Cancer Center, University of Florida, Gainesville, FL

    William L. Blalock

  3. BD Biosciences Clontech, Palo Alto, CA

    Xiao-Yang Wang

  4. Department of Biology, East Carolina University, Greenville, NC

    Julianne M. Davis

  5. Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson College of Medicine, Philadelphia, PA

    Martyn K. White

Authors
  1. Linda S. Steelman
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  2. William L. Blalock
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  3. Xiao-Yang Wang
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  4. Phillip W. Moye
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  5. John T. Lee
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  6. John G. Shelton
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  7. Patrick M. Navolanic
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  8. Julianne M. Davis
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  9. Steven L. Knapp
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  10. Richard A. Franklin
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  11. Martyn K. White
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  12. James A. McCubrey
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Editor information

Editors and Affiliations

  1. Brody School of Medicine, East Carolina University, Greenville, NC

    David M. Terrian

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© 2003 Humana Press Inc., Totowa, NJ

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Steelman, L.S. et al. (2003). Elucidation of Signal Transduction Pathways by Transfection of Cells with Modified Oncogenes. In: Terrian, D.M. (eds) Cancer Cell Signaling. Methods in Molecular Biology™, vol 218. Humana Press. https://doi.org/10.1385/1-59259-356-9:203

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  • DOI: https://doi.org/10.1385/1-59259-356-9:203

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-075-5

  • Online ISBN: 978-1-59259-356-9

  • eBook Packages: Springer Protocols

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