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Lentivirus Vector-Mediated Gene Transfer to Cardiomyocytes

  • Protocol
Cardiac Cell and Gene Transfer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 219))

Abstract

Gene therapy has the potential to reverse the genetic causes and modify the pathophysiology of many innate and acquired diseases (1–4). Transduction of foreign DNA into cardiac myocytes is of potential value for therapeutic applications (5,6) and also offers an experimental approach to investigate the roles of individual genes in cardiovascular pathophysiology. Both efficient delivery and long-term expression of transduced genes is required before the full benefit of genetic manipulation strategies can be realized in the cardiovascular system. However, all the current methods of gene delivery have major limitations.

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Author information

Authors and Affiliations

  1. Institute for Genetic Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA

    Tsuyoshi Sakoda, Noriyuki Kasahara & Larry Kedes

  2. Department of Pathology, Keck School of Medicine, University of Southern California, Los Angeles, CA

    Noriyuki Kasahara

  3. Department of Biochemistry and Molecular Biology, Keck School of Medicine, University of Southern California, Los Angeles, CA

    Larry Kedes

Authors
  1. Tsuyoshi Sakoda
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  2. Noriyuki Kasahara
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  3. Larry Kedes
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Editor information

Editors and Affiliations

  1. Department of Physiology, Department of Internal Medicine, University of Michigan, Ann Arbor, MI

    Joseph M. Metzger (Director) (Director)

  2. Center for Integrative Genomics, University of Michigan, Ann Arbor, MI

    Joseph M. Metzger (Director) (Director)

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© 2003 Humana Press Inc.

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Sakoda, T., Kasahara, N., Kedes, L. (2003). Lentivirus Vector-Mediated Gene Transfer to Cardiomyocytes. In: Metzger, J.M. (eds) Cardiac Cell and Gene Transfer. Methods in Molecular Biology, vol 219. Springer, Totowa, NJ. https://doi.org/10.1385/1-59259-350-X:53

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  • DOI: https://doi.org/10.1385/1-59259-350-X:53

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-0-89603-994-0

  • Online ISBN: 978-1-59259-350-7

  • eBook Packages: Springer Protocols

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