Abstract
Trichinella worms (family: Trichinellidae; phylum: Nematoda) are parasites that mainly infect mammals, including humans, although they have been found in birds and, recently, in African crocodiles (1,2). The main reservoir is represented by carnivores with cannibalistic and scavenger behavior. These parasites are widespread on all continents but Antarctica, from frigid to torrid zones. The main distinguishing feature of their life cycle is that two generations occur in the same host. The first generation (from L1 larva to adult) is present in the gut and the second generation (from a newborn larva of 80 μm in length to an infective larva of 0.6-1.0 mm in length) is present in the cell of striated muscles that is modified by the larva (referred to as the nurse cell) (3,4).
Keywords
- Nurse Cell
- Dissection Microscope
- Digestion Fluid
- Single Larva
- Conical Vial
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, access via your institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsSpringer Nature is developing a new tool to find and evaluate Protocols. Learn more
References
Murrell, K.D., Lichtenfels, R.J., Zarlenga, D.S., and Pozio, E. (2000) The system-atics of Trichinella with a key to species. Vet. Parasitol. 93, 293–307.
Pozio, E. (2001) New patterns of Trichinella infections. Vet. Parasitol. 98, 133–148.
Despommier, D.D. (1998) How does Trichinella spiralis make itself at home? Parasitol. Today 14, 318–323.
Ljungström, I., Murrell, D., Pozio, E., and Wakelin, D. ( 1998) Trichinellosis, in Zoonoses. Biology, Clinical Practice, and Public Health Control (Palmer, S.R., Lord Soulsby, and Simpson, D.I.H., ), Oxford University Press, Oxford, UK, pp. 789–802.
Pozio, E. (1998) Trichinellosis in the European Union: Epidemiology, ecology and economic impact. Parasitol. Today 14, 35–38.
Pozio, E., Casulli, A., Bologov, V.V., Marucci, G., and La Rosa, G. (2002). Hunting practices increase the prevalence of Trichinella infection in wolves from European Russia. J. Parasitol. 87, 1498–1501.
Pozio, E., Sacchini, D., Sacchi, L., Tamburrini, A., and Alberici, F. (2001). Failure of mebendazole in the treatment of humans with Trichinella spiralis infection at the stage of encapsulating larvae. Clin. Infect. Dis. 32, 638–642.
Pozio, E. (2000). Factors affecting the flow among domestic, synanthropic and sylvatic cycles of Trichinella. Vet. Parasitol. 93, 241–262.
Zarlenga, D.S., Chute, M.B., Martin, A., and Kapel, C. M. O. (1999) A single multiplex PCR for unequivocal differentiation of six distinct genotypes of Trichinella and three geographical genotypes of Trichinella pseudospiralis. Int. J. Parasitol. 29, 1859–1867.
Pozio, E., Owen, I.L., La Rosa, G., Sacchi, L., Rossi, P., and Corona, S. (1999) Trichinellapapuae n. sp. (Nematoda), a new non-encapsulated species from domestic and sylvatic swine of Papua New Guinea. Int. J. Parasitol. 29, 1825–1839.
Wu, Z., Nagano, I., Pozio, E., and Takahashi, Y. (1999) Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for the identification of Trichinella isolates. Parasitology 118, 211–218.
Pozio, E., La Rosa, G., and Rossi, P. (1989) Trichinella reference centre. Parasitol. Today 5, 169–170.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2003 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Pozio, E., La Rosa, G. (2003). PCR-Derived Methods for the Identification of Trichinella Parasites from Animal and Human Samples. In: Sachse, K., Frey, J. (eds) PCR Detection of Microbial Pathogens. Methods in Molecular Biology™, vol 216. Humana Press. https://doi.org/10.1385/1-59259-344-5:299
Download citation
DOI: https://doi.org/10.1385/1-59259-344-5:299
Publisher Name: Humana Press
Print ISBN: 978-1-58829-049-6
Online ISBN: 978-1-59259-344-6
eBook Packages: Springer Protocols