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Detection and Differentiation of Chlamydiae by Nested PCR

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PCR Detection of Microbial Pathogens

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 216))

Abstract

Chlamydiae are obligate intracellular prokaryotes with a hexalaminar cell wall that, in contrast to other gram-negative bacteria, contains no peptidoglycan. As a major antigenic constitutent, their outer membrane contains a 10-kDa lipopolysaccharide (LPS) with a trisaccharide epitope specific for the family Chlamydiaceae (1). Another characteristic antigenic component is the major outer membrane protein (MOMP), a cysteine-rich protein of approx 40 kDa representing approx 60% of the weight of the outer membrane. This molecule harbors several genus- and species-specific antigenic determinants in the conserved regions and serovar-specific epitopes in variable domains (2).

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Author information

Authors and Affiliations

  1. Division 4: Bacterial Animal Diseases and Control of Zoonoses, Federal Institute for Health Protection of Consumers and Veterinary Medicine (BgVV), Jena, Germany

    Konrad Sachse & Helmut Hotzel

Authors
  1. Konrad Sachse
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  2. Helmut Hotzel
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Editor information

Editors and Affiliations

  1. Federal Institute for Health Protection of Consumers and Veterinary Medicine (BgVV), Jena, Germany

    Konrad Sachse (Dr. Rer. Nat.) (Dr. Rer. Nat.)

  2. University of Bern, Bern, Switzerland

    Joachim Frey PhD

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© 2003 Humana Press Inc., Totowa, NJ

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Sachse, K., Hotzel, H. (2003). Detection and Differentiation of Chlamydiae by Nested PCR. In: Sachse, K., Frey, J. (eds) PCR Detection of Microbial Pathogens. Methods in Molecular Biology™, vol 216. Humana Press. https://doi.org/10.1385/1-59259-344-5:123

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  • DOI: https://doi.org/10.1385/1-59259-344-5:123

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-049-6

  • Online ISBN: 978-1-59259-344-6

  • eBook Packages: Springer Protocols

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