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Generating Conditional Mouse Mutants via Tetracycline-Controlled Gene Expression

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Transgenic Mouse

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 209))

Abstract

With the tetracycline (Tc)-controlled gene expression system (Tet system), an experimental approach has been developed that permits alteration of individual gene activities in complex genetic systems in a truly conditional fashion, i.e., it is temporally defined, quantitative, and reversible. Monitoring the phenotypic changes that accompany such highly defined alterations has provided new insights into numerous biologic processes hereto not amenable to genetic dissection. The various versions of the Tet system (1–3) were successfully incorporated into a variety of model organisms such as Streptomyces cerevisiae, Dictyostelium, Drosophila, Arabidopsis, moss, mice, and rats (for review, see refs. 3–5).

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Authors
  1. Kai Schönig
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  2. Hermann Bujard
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Editor information

Editors and Affiliations

  1. Department of Molecular Genetics, University of Maastricht, Maastricht, The Netherlands

    Marten H. Hofker

  2. Department of Pediatrics and Adolescent Medicine, Mayo Clinic, Rochester, MN

    Jan van Deursen

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© 2003 Humana Press Inc.

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Schönig, K., Bujard, H. (2003). Generating Conditional Mouse Mutants via Tetracycline-Controlled Gene Expression. In: Hofker, M.H., van Deursen, J. (eds) Transgenic Mouse. Methods in Molecular Biology™, vol 209. Humana Press. https://doi.org/10.1385/1-59259-340-2:69

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  • DOI: https://doi.org/10.1385/1-59259-340-2:69

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-915-5

  • Online ISBN: 978-1-59259-340-8

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