Abstract
Wound healing disorders present a serious clinical problem and are likely to increase since they are associated with diseases such as diabetes, hypertension, and obesity. Additionally, increasing life expectancies will cause more people to face such disorders and further aggravate this medical problem. Thus, several animal models have been established to serve as an experimental basis to determine molecular and cellular mechanisms underlying and controlling an undisturbed healing process. Here we describe a model of excisional skin wounding in mice that can be used to assess molecular, cellular, and tissue movements in healthy mice as well as in mouse models characterized by impaired or altered healing conditions such as genetically deficient or transgenic animals. Moreover, we point out that the presented model of excisional skin wounding can be easily adapted from a basic experimental model to a model that deals with more detailed questions of interest.
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References
Clark, R. A. F. (1996) Wound repair: overview and general considerations, in The Molecular and Cellular Biology of Wound Repair (Clark, R. A. F., ed.), Plenum, New York, pp. 3–50.
Singer, A. J. and Clark, R. A. F. (1999) Cutaneous wound healing. N. Engl. J. Med. 341, 738–746.
Martin, P. (1997) Wound healing—aiming for perfect skin regeneration. Science 276, 75–81.
Wetzler, C., Kämpfer, H., Stallmeyer, B., Pfeilschifter, J., and Frank, S. (2000) Large and sustained induction of chemokines during impaired wound healing in the genetically diabetic mouse: prolonged persistence of neutrophils and macrophages during the late phase of repair. J. Invest. Dermatol. 115, 245–253.
Yamasaki, K., Edington, H. D. J., McClosky, C., Tzeng, E., Lizonova, A., Kovesdi, I., Steed, D. L., and Billiar, T. R. (1998) Reversal of impaired wound repair in iNOS-deficient mice by topical adenoviral-mediated iNOS gene transfer. J. Clin. Invest. 101, 967–971.
Stallmeyer, B., Kämpfer, H., Kolb, N., Pfeilschifter, J., and Frank, S. (1999) The function of nitric oxide in wound repair: inhibition of inducible nitric oxide-synthase severely impairs wound reepithelialization. J. Invest. Dermatol. 113, 1090–1098.
Frank, S., Stallmeyer, B., Kämpfer, H., Kolb, N., and Pfeilschifter, J. (2000) Leptin enhances wound re-epithelialization and constitutes a direct function of leptin in skin repair. J. Clin. Invest. 106, 501–509.
Chomczynski, P. and Sacchi, N. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162, 156–159.
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© 2003 Humana Press Inc., Totowa, NJ
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Frank, S., Kämpfer, H. (2003). Excisional Wound Healing. In: DiPietro, L.A., Burns, A.L. (eds) Wound Healing. Methods in Molecular Medicine™, vol 78. Humana Press, Totowa, NJ. https://doi.org/10.1385/1-59259-332-1:003
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DOI: https://doi.org/10.1385/1-59259-332-1:003
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-999-5
Online ISBN: 978-1-59259-332-3
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