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Tumor Suppressor Genes pp 3–15Cite as

Utilizing NMR to Study the Structure of Growth-Inhibitory Proteins

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 223))

Abstract

The underlying premise of structural biology is that the fundamental understanding of biological functions lies in the three-dimensional structures of proteins and other biopolymers. The two well-established experimental methods for determining the high-resolution structures of proteins have both contributed to the wealth of structural information available for the tumor suppressor genes. The tumor suppressor proteins whose structures have been determined by nuclear magnetic resonance (NMR) spectroscopy are listed in Table 1. Although X-ray crystallography plays a central role in high-throughput structure determination in the current structural genomics efforts, several features of NMR spectroscopy make it extremely well suited for three-dimensional structure determination as well as for the structure-function analysis of proteins (1,2).

Table 1 Tumor Suppressor Proteins Whose Structures Have Been Determined by NMR Spectroscopy in Solution, with Protein Data Bank Identification (PDB ID) Codes (http://www.rcsb.org/pdb/)
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Authors and Affiliations

  1. The Burnham Institute, La Jolla, CA

    Francesca M. Marassi

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  1. Francesca M. Marassi
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Editors and Affiliations

  1. Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA

    Wafik S. El-Deiry MD, PhD

  2. Department of Genetics, University of Pennsylvania School of Medicine, Philadelphia, PA

    Wafik S. El-Deiry MD, PhD

  3. Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, PA

    Wafik S. El-Deiry MD, PhD

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© 2003 Humana Press Inc.

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Marassi, F.M. (2003). Utilizing NMR to Study the Structure of Growth-Inhibitory Proteins. In: El-Deiry, W.S. (eds) Tumor Suppressor Genes. Methods in Molecular Biology™, vol 223. Humana Press. https://doi.org/10.1385/1-59259-329-1:3

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  • DOI: https://doi.org/10.1385/1-59259-329-1:3

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-987-2

  • Online ISBN: 978-1-59259-329-3

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