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Somatic Cell Knockouts of Tumor Suppressor Genes

  • Protocol
Tumor Suppressor Genes

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 223))

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Abstract

Gene targeting is the modification of specific DNA sequences in a living organism. Three requirements must be met in order for gene targeting to be successful (1). The process must be directed, so that it affects only the locus of choice. The targeting procedure requires specificity, such that a predetermined sequence can be inserted or substituted at the target locus. Finally, the process should be efficient.

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Author information

Authors and Affiliations

  1. Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, RI

    Ruth Hemmer, Wenyi Wei, Annie Dutriaux & John M. Sedivy

Authors
  1. Ruth Hemmer
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  2. Wenyi Wei
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  3. Annie Dutriaux
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  4. John M. Sedivy
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Editor information

Editors and Affiliations

  1. Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA

    Wafik S. El-Deiry MD, PhD

  2. Department of Genetics, University of Pennsylvania School of Medicine, Philadelphia, PA

    Wafik S. El-Deiry MD, PhD

  3. Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, PA

    Wafik S. El-Deiry MD, PhD

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© 2003 Humana Press Inc.

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Hemmer, R., Wei, W., Dutriaux, A., Sedivy, J.M. (2003). Somatic Cell Knockouts of Tumor Suppressor Genes. In: El-Deiry, W.S. (eds) Tumor Suppressor Genes. Methods in Molecular Biology™, vol 223. Humana Press. https://doi.org/10.1385/1-59259-329-1:187

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  • DOI: https://doi.org/10.1385/1-59259-329-1:187

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-987-2

  • Online ISBN: 978-1-59259-329-3

  • eBook Packages: Springer Protocols

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