Abstract
Small cell lung cancer (SCLC) comprises a significant fraction of all lung cancers; it is most frequent in women, where it represents up to 25% (1). It is characterized by neuroendocrine differentiation, with immunohistochemistry for certain neuroepithelial markers used in diagnosis to differentiate SCLC from other types of lung cancer. We have evaluated the use of reverse transcription polymerase chain reaction (RT-PCR) for selected neuroendocrine markers as a way to specifically detect SCLC cells in (1) surgical, postmortem or bronchoscopic biopsies and (2) the peripheral blood or lymph node aspirates from SCLC patients. Products are detected either directly by ethidium bromide staining of agarose gels or, with enhanced sensitivity, by transfer to a membrane followed by hybridization with a specific radiolabeled probe.
Keywords
- Sodium Dodecyl Sulphate
- Reverse Transcription Polymerase Chain Reaction
- Small Cell Lung Cancer
- Small Cell Lung Cancer Cell
- Small Cell Lung Cancer Patient
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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© 2003 Humana Press Inc., Totowa, NJ
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Coulson, J.M., Ahmed, S.I., Quinn, J.P., Woll, P.J. (2003). Detection of Small Cell Lung Cancer by RT-PCR for Neuropeptides, Neuropeptide Receptors, or a Splice Variant of the Neuron Restrictive Silencer Factor. In: Driscoll, B. (eds) Lung Cancer. Methods in Molecular Medicine™, vol 75. Humana Press, Totowa, NJ. https://doi.org/10.1385/1-59259-324-0:335
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DOI: https://doi.org/10.1385/1-59259-324-0:335
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-920-9
Online ISBN: 978-1-59259-324-8
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