Abstract
Genetic tools are necessary to unravel complex phenotypes, like the formation of attaching and effacing lesions by Shiga toxin-producing Escherichia coli (STEC). To inactivate a particular gene, we use a precise and convenient method that is based on the generation of an in-frame deletion within this gene. The advantages of this technique in comparison with others (e.g., transposon mutagenesis or site-directed insertional mutagenesis) are as follows:
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No polar effects on the transcription of downstream located genes by disrupting polycistronic RNA transcripts.
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Genetic stability of the generated deletion mutants, because a reversion to the wild-type is impossible.
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No continuous selective pressure is needed to maintain the mutant.
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© 2003 Humana Press Inc.
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Djafari, S., Hauf, N.D., Tyczka, J.F. (2003). Generation of Isogenic Deletion Mutants of STEC. In: Philpott, D., Ebel, F. (eds) E. coli. Methods in Molecular Medicine™, vol 73. Humana Press. https://doi.org/10.1385/1-59259-316-X:113
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DOI: https://doi.org/10.1385/1-59259-316-X:113
Publisher Name: Humana Press
Print ISBN: 978-0-89603-939-1
Online ISBN: 978-1-59259-316-3
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