High-Throughput Purification of PolyHis-Tagged Recombinant Fusion Proteins

  • Thomas Lanio
  • Albert Jeltsch
  • Alfred Pingoud
Part of the Methods in Molecular Biology™ book series (MIMB, volume 205)


Methods for the efficient overexpression and purification of recombinant proteins are of paramount importance for biotechnology. In particular, for the era of functional genomics that we have entered after sequencing complete genomes, this has become a routine matter. High-throughput protein purification will, therefore, become a key technology to unravel the function of gene products (Fig. 1). To facilitate the procedure of protein purification, several tags to generate fusion proteins are available (e.g., polyHis, GST, MBP, CBP, and the like) for parallel purification using matrices coupled with affinity anchors, like Ni2+-nitrilotriacetic acid (Ni2+-NTA) which is a powerful chelating ligand for the purification of His6-tagged proteins under native conditions. Ni-NTA affinity matrices allow to purify the protein of interest contained in a crude protein mixture at a concentration of 1% in one step to more than 95% homogeneity (1).
Fig. 1.

Schematic overview of the high-throughput protein purification method. Cells are grown, harvested, and lysed in 1-mL square well blocks. The His6-tagged variants are isolated by transferring the lysate to Ni-NTA coated microplates from which they are eluted after washing. A 1.5-mL cell culture typically yields 5-10 pmol recombinant protein.


Elution Buffer Protein Purification Nitrilotriacetic Acid Refrigerate Centrifuge Purify Enzyme Preparation 
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  1. 1.
    Janknecht, R., de Martynoff, G., Lou, J., Hipskind, R. A., Nordheim, A., and Stunnenberg, H. G. (1991) Rapid and efficient purification of native histidine-tagged protein expressed by recombinant vaccinia virus. Proc. Natl. Acad. Sci.USA 88, 8972–8976.PubMedCrossRefGoogle Scholar
  2. 2.
    Arnold, F. H. and Volkov, A. A. (1999) Directed evolution of biocatalysts. Curr.Opin. Chem. Biol. 3, 54–59.PubMedCrossRefGoogle Scholar
  3. 3.
    Benner S. A. (1993) Catalysis: design versus selection. Science 261, 1402–1403.PubMedCrossRefGoogle Scholar
  4. 4.
    Moore, J. C. and Arnold, F. H. (1996) Directed evolution of a para-nitrobenzyl esterase for aqueous-organic solvents. Nat.Biotechnol. 14, 458–467.PubMedCrossRefGoogle Scholar
  5. 5.
    Crameri, A., Raillard, S. A., Bermudez, E., and Stemmer W. P. (1998) DNA shuffling of a family of genes from diverse species accelerates directed evolution. Nature 391, 288–291.PubMedCrossRefGoogle Scholar
  6. 6.
    Lanio, T., Jeltsch, A., and Pingoud, A. (2000) Automated purification of His6-tagged proteins allows exhaustive screening of libraries generated by random mutagenesis. Biotechniques 29, 338–342.PubMedGoogle Scholar
  7. 7.
    Meier-Ewert, S., Maier, E., Ahmadi, A., Curtis, J., and Lehrach, H. (1993) An automated approach to generating expressed sequence catalogues. Nature 361, 375–376.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 2003

Authors and Affiliations

  • Thomas Lanio
    • 1
  • Albert Jeltsch
    • 1
  • Alfred Pingoud
    • 1
  1. 1.Institut für BiochemieJustus-Liebig-UniversitätGiessenGermany

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