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Multi-Telomere FISH

  • Samantha J. L. Knight
  • Jonathan Flint
Part of the Methods in Molecular Biology™ book series (MIMB, volume 204)

Abstract

The standard investigation for suspected chromosomal rearrangements in patients is cytogenetic analysis at a 400–550 band resolution, yet this cannot routinely detect rearrangements smaller than 5 Megabases (Mb), and much larger abnormalities escape notice if they occur in regions where the banding pattern is not distinctive. In the future, this problem will largely be solved by the use of high resolution micro-arrays that will allow the entire genome to be investigated for submicroscopic chromosomal rearrangements. However, until this technology becomes routine, the only way of achieving increased reliability and resolution is to focus on specific chromosomal regions such as the ends of chromosomes (telomeres).

Keywords

Wash Solution Heating Block Telomere Probe Spontaneous Recurrent Miscarriage Subtelomeric Rearrangement 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 2002

Authors and Affiliations

  • Samantha J. L. Knight
    • 1
  • Jonathan Flint
    • 2
  1. 1.John Radcliffe HospitalHeadingtonUK
  2. 2.Welcome Trust Center for Human GeneticsChurchill HospitalHeadingtonUK

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