Abstract
Zone electrophoresis is the basic mode of capillary electrophoresis, and it allows separation of ions having different charge-to-size ratios. Carbohydrates having ionic groups can also be the objects of separation by this mode. A typical example is the separation of carboxylated/sulfated oligosaccharides from glycosaminoglycans, as described in Chapter 15. Most carbohydrates, however, have no electric charge under normal conditions. Therefore, introduction of ionic tags plays an important role in zone electrophoresis of such carbohydrates.
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References
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Honda, S., Suzuki, S. (2003). Separation of Oligosaccharides by Capillary Electrophoresis Using Buffer Modifiers. In: Thibault, P., Honda, S. (eds) Capillary Electrophoresis of Carbohydrates. Methods in Molecular Biology™, vol 213. Humana Press. https://doi.org/10.1385/1-59259-294-5:93
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DOI: https://doi.org/10.1385/1-59259-294-5:93
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