Abstract
The development of general chemical approaches for controlling gene expression would facilitate investigations into the details of cellular function (1). Antisense oligonucleotides have been widely used to block the expression of genes or alter splicing (2), but their use has suffered from questions regarding their potency and specificity. In addition, successful antisense experiments often require a large effort to identify oligomers with adequate activity (3). Peptide nucleic acids (PNAs) bind with exceptionally high affinity to complementary sequences and possess an uncharged backbone that is unlikely to prompt interactions with the many cellular proteins that bind anionic macromolecules (4). It is reasonable to hypothesize, therefore, that PNAs may possess advantages as antisense agents. In addition, PNAs have an unmatched ability to invade duplex DNA, suggesting that they may bind to chromosomal targets and act as antigene agents (4).
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Braasch, D.A., Corey, D.R. (2002). Lipid-Mediated Introduction of Peptide Nucleic Acids Into Cells. In: Nielsen, P.E. (eds) Peptide Nucleic Acids. Methods in Molecular Biology, vol 208. Springer, Totowa, NJ. https://doi.org/10.1385/1-59259-290-2:211
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DOI: https://doi.org/10.1385/1-59259-290-2:211
Publisher Name: Springer, Totowa, NJ
Print ISBN: 978-0-89603-976-6
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