Abstract
The green fluorescent protein (GFP) from the jellyfish Aequorea victoria has become one of the most important markers for studying gene expression and protein targeting in intact cells and organisms (1–3). GFP represents the first genetically encoded reporter molecule that is detectable in the absence of an enzymatic substrate or cofactor, in a variety of cell types.
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Topell, S., Glockshuber, R. (2002). Circular Permutation of the Green Fluorescent Protein. In: Hicks, B.W. (eds) Green Fluorescent Protein. Methods in Molecular Biology, vol 183. Humana Press. https://doi.org/10.1385/1-59259-280-5:031
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DOI: https://doi.org/10.1385/1-59259-280-5:031
Publisher Name: Humana Press
Print ISBN: 978-0-89603-905-6
Online ISBN: 978-1-59259-280-7
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