Abstract
Recently, we cloned six new green fluorescent protein (GFP)-like fluorescent proteins from five species of Antozoa (1), including one red-emitting variant (DsRed), which is now commercially available. This project did not require expeditions and collection of animals on reefs: In all cases, the starting material was just several milligrams of tissue (e.g., a tentacle tip of a sea anemone), collected from a specimen in a private aquarium. This truly noninvasive kind of study was possible because the approach of total cDNA amplification, which is extensively applied to various tasks and biological models in our lab. This chapter outlines several year’s of experience in this helpful technique.
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Matz, M.V. (2002). Amplification of Representative cDNA Samples from Microscopic Amounts of Invertebrate Tissue to Search for New Genes. In: Hicks, B.W. (eds) Green Fluorescent Protein. Methods in Molecular Biology, vol 183. Humana Press. https://doi.org/10.1385/1-59259-280-5:003
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DOI: https://doi.org/10.1385/1-59259-280-5:003
Publisher Name: Humana Press
Print ISBN: 978-0-89603-905-6
Online ISBN: 978-1-59259-280-7
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