PCR Mutation Detection Protocols pp 47-50 | Cite as
Allele-Specific Oligonucleotide PCR
Protocol
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Abstract
Many single-base substitutions that lead to inherited diseases, the predisposition to genetic disorders, and cancer are increasingly being discovered. The ability to amplify specific DNA sequences by the polymerase chain reaction (PCR) (1) has made it possible to rapidly and accurately diagnose many inheritable diseases.
Keywords
Polymerase Chain Reaction Reaction Amplification Refractory Mutation System Inheritable Disease Common Primer Polymerase Chain Reaction Reaction Buffer
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References
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© Humana Press Inc., Totowa, NJ 2002