Abstract
Because it has been 25 yr since the successful cultivation of Plasmodium falciparum (1), most researchers do not remember how difficult it was to work with malaria parasites, especially in vitro. Before the development of current methods, malaria parasite cultures were always short-term, lasting only a few days with decreasing numbers until the parasites died out. Not only was it extremely inconvenient to constantly begin new cultures, but investigators worked with parasites that were abnormal in the sense that the overall population was dying. Sixty-four years passed between the initial studies of Bass and Johns (2) and the successful development of continuous cultures of P. falciparum. During that time, many reported advances were able to extend the time of cultivation by only a few days but always with the inevitable terminal results. Most of the early investigators on in vitro cultivation used the bird malaria parasite P. lophurae or the simian parasite P. knowlesi, but the first successful cultivation of any malarial parasite was P. falciparum, the most important of the human malaria species. Using essentially the same procedures, other species of malarial parasites now have been cultured, including P. fragile, P. inui, and P. cynomolgi, but these species offer no real advantages over the use of P. falciparum. Methods for the cultivation of P. vivax have been reported but these cultures apparently require a continuous source of human reticulocytes which limits the suitability and presents a nearly insurmountable barrier to all but a few laboratories (3). A recent review of the impact of continuous cultures of P. falciparum underscores the tremendous contributions of this technique on malaria research (4).
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References
Trager, W. and Jensen, J. B. (1976) Human malaria parasites in continuous culture. Science 193, 673ā675.
Bass, C. C. and Johns, F. M. (1912) The cultivation of malarial plasmodia (Plasmodium vivax and Plasmodium falciparum) in vitro. J. Exp. Med. 16, 567ā579.
Golenda, C. F., Li, J., and Rosenberg, R. (1997) Continuous in vitro propagation of the malaria parasite Plasmodium vivax. Proc. Natl. Acad. Sci. USA 94, 6786ā6791.
Trager, W. and Jensen, J. B. (1997) Continuous culture of Plasmodium falciparum. Its impact on malaria research. Int. J. Parasitol. 27, 989ā1006.
Trager, W. and Jensen, J. B. (1978) Cultivation of malarial parasites, a review. Nature 273, 621,622.
Jensen, J. B. and Trager, W. (1977) Plasmodium falciparum in culture: use of outdated erythrocytes and description of the candle jar method. J. Parasitol. 63, 883ā886.
Jensen, J. B. (1978) Concentration of trophozoite-and schizont-infected erythrocytes of Plasmodium falciparum from continuous cultures. Am. J. Trop. Med. Hyg. 27, 1274ā1276.
Rowe, A. W., Eyster, E., and Kellner, A. (1968) Liquid nitrogen preservation of red blood cells for transfusion. Cryobiology 5, 119ā128.
Lambros, C. and Vanderberg, J. P. (1979) Synchronization of Plasmodium falciparum erythrocyte stages in cultures. J. Parasitol. 65, 418ā420.
Vernes, A., Haynes, J. D., Tapchaisri, P., Williams, J. L., Dutoit, E., and Diggs, C. (1984) Plasmodium falciparum strain-specific human antibody inhibits merozoite invasion of erythrocytes. Am. J. Trop. Med. Hyg. 33, 197ā203.
Pasvol, G., Wilson, R. J. M., Smalley, M. E., and Brown, J. (1978) Separation of viable schizontinfected red cells of Plasmodium falciparum from human blood. Ann. Trop. Med. Parasitol. 72, 87,88.
Divo, A. A., Geary, T. G., Davis, N. L., and Jensen, J. B. (1985) Nutritional requirements of Plasmodium falciparum in culture. I: exogenously supplied dialyzable components necessary for continuous growth. J. Protozool. 32, 59ā64.
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Ā© 2002 Humana Press, Inc., Totowa, NJ
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Jensen, J.B. (2002). In Vitro Culture of Plasmodium Parasites. In: Doolan, D.L. (eds) Malaria Methods and Protocols. Methods in Molecular Medicineā¢, vol 72. Humana Press. https://doi.org/10.1385/1-59259-271-6:477
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DOI: https://doi.org/10.1385/1-59259-271-6:477
Publisher Name: Humana Press
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