Abstract
Vaccination is the most cost-effective measure to control the pathological effects of an infectious agent (1,2). With the combined use of molecular cloning and of modern sequencing methods, the sequence of many protein components present in different infectious agents have been elucidated, opening the way to the design and development of various vaccination strategies which include peptides, proteins and DNA. Peptide vaccination is per se the most simple, straightforward, and, in our opinion, safest approach to vaccination of the world population. For peptide, we intend protein fragments of any length obtained from chemical synthesis (3-5). One drawback of using short peptides is the limitation of the intervention to defined members of the population that carry the major histocompatibility complex (MHC) antigen(s) which the peptides bind to (MHC restriction). This limitation can, in principle, be overcome by using a physical or chemical mixture of short peptides or to lengthen the size of the peptide fragment in order to cover the MHC antigens of the entire population (6,7).
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References
Gander, B., Merkle, H. P., and Corradin, G. (eds.) (1997) Antigen Delivery System. Harwood Academic Publishers, Amsterdam, The Netherlands.
State of the Worldās Vaccines and Immunization. WHO/UNICEF documen. (1996) WHO/GPV/ 96.04.
Merrifield, R. B. (1986) Solid phase synthesis. Science 232, 341ā374.
Atherton, E., Logan, C. J., and Shepard, R. C. (1988) Peptide synthesis. Part II. Procedures for solid phase synthesis using Na-fluorenylmethoxycarbamylamino-acids on polyamide supports: synthesis of substance P and of acyl carrier protein 65-74 decapeptides. J. Chem. Soc. (Lond.) 1, 538.
Pennington, M. W. and Dunn, B. M. (eds.) (1994) Peptide Synthesis Protocols. Methods in Molecular Biology, vol. 35. Humana Press, Totowa, NJ.
Sinigaglia, F., Guttinger, M., Romagnoli, P., and Takacs, B. (1990) Malaria antigens and MHC restriction. Immunol. Lett. 25, 265ā270.
Hoffman, S. L., Berzofsky, J. A., Isenbarger, D., Zeltser, E., Majarian, W. R., Gross, M., and Ballou, W. R. (1989) Immune response gene regulation of immunity to Plasmodium berghei sporozoites and circumsporozoite protein vaccines. Overcoming genetic restriction with whole organism and subunit vaccines. J Immunol. 142, 3581ā3584.
Heimbrook, D. C., Saari, W. S., Balishin, N. L., Fisher, T. W., Friedman, A., Kiefer, D. M., et al. (1991) Gastrin releasing peptide antagonists with improved potency and stability. J Med. Chem. 34, 2102ā2107.
Valmori, D., Pessi, A., Bianchi, E., and Corradin, G. (1992) Use of human universal antigenic tetanus toxin T cell epitopes as carriers for human vaccination. J Immunol. 149, 717ā721.
Renggli, J., Valmori, D., Romero, J. F., Eberl, G., Romero, P., Betschart, B., and Corradin, G. (1995) CD8+T-cell protective immunity induced by immunization with Plasmodium berghei CS protein-derived synthetic peptides: evidence that localization of peptide-specific CTL is crucial for protection against malaria. Immunol. Lett. 46, 199ā205.
Migliorini, P., Betschart, B., and Corradin, G. (1993) Malaria vaccine: immunization of mice with a synthetic T cell helper epitope leads to protective immunity. Eur. J. Immunol. 23, 582ā585.
Blum-Tirouvanziam, U., Beghdadi-Rais, C., Roggero, M. A., Valmori, D., Bertholet, S., Bron, C., Fasel, N., and Corradin, G. (1995) Elicitation of specific cytotoxic T cells by immunization with malaria soluble synthetic polypeptides. J Immunol. 153, 4134ā4141.
Tam, J. P., Clavijo, P., Lu, Y., Nussenzweig, V., Nussenzweig, R., and Zavala, F. (1990) Incorporation of T and B epitopes of the circumsporozoite protein in a chemically defined synthetic vaccine against malaria. J Exp. Med. 171, 299ā306.
Wang, R., Charoenvit, Y., Corradin, G., Porrozzi, R., Hunter, R. L., Glenn, G., et al. (1995) Induction of protective polyclonal antibodies by immunization with a Plasmodium yoelii circumsporozoite protein multiple antigen peptide vaccine. J Immunol. 154, 2784ā2793.
Wang, R., Charoenvit, Y., Corradin, G., De La Vega, P., Franke, E. D., and Hoffman, S. L. (1996) Protection against malaria by Plasmodium yoelii sporozoite surface protein 2 linear peptide induction of CD4+T cell-and IFN-g-dependant elimination of infected hepatocytes. J Immunol. 157, 4061ā4067.
Porath, J. and Flodin, P. (1959) Gel filtration: a method for desalting and group separation. Nature 183, 1657ā1659.
Walker, J. M. (ed.) (1994) Basic Protein and Peptide Protocols. Methods in Molecular Biology, vol.32. Humana Press, Totowa, NJ.
Dunn, B. M. and Pennington, M. W. (eds.) (1994) Peptide Analysis Protocols. Methods in Molecular Biology, vol. 36. Humana Press, Totowa, NJ.
Smith, J. B., Thevenon-Emeric, G., Smith, D. L., and Green, B. (1991) Elucidation of the primary structures of proteins by mass spectrometry. Anal. Biochem. 193, 118ā124.
Stachowiak, K., et al. (1988) Rapid protein sequencing by the enzyme-thermospray LC/MS method. J. Amer. Chem. Soc. 110, 1758ā1765.
Stachowiak, K., Otlewski, J., Polanowski, A., and Dyckes, D. F. (1990) Monitoring protein cleavage and concurrent disulfide bond assignment using thermospray LC/MS. Pept. Res. 3, 148ā154.
Edelhoch, H. (1967) Spectroscopic determination of tryptophan and tyrosine in proteins. Biochemistry 6, 1948ā1954.
Migliorini, P., Boulanger, N., Betschard, B., and Corradin, G. (1990) Plasmodium berghei subunit vaccine: repeat synthetic peptide of circumsporozoite protein comprising T-and B-cell epitopes fail to confer immunity. Scand. J. Immun. 31, 237ā242.
Perlaza, B. L., Arevalo-Herrera, M., Brahimi, K., Quintero, G., Palomino, J. C., Gras-Masee, H., et al. (1998) Immunogenicity of four Plasmodium falciparum preerythrocytic antigens in Aotus lemurinus monkeys. Infect. Immun. 66, 3423ā3428.
Franke, E. D., Corradin, G., and Hoffman, S. L. (1997) Induction of protective CTL responses against the Plasmodium yoelii circumsporozoite protein by immunization with peptides. J. Immunol. 159, 3424ā3433.
Lawrence, G. W., Saul, A., Giddy, A. J., Kemp, R., and Pye, D. (1997) Phase I trial in humans of an oil-based adjuvant SEPPIC MONTANIDE ISA 720. Vaccine 15, 176ā178.
Pye, D., Vandenberg, K. L., Dyer, S. L., Irving, D. O., Goss, N. H., Woodrow, G. C., et al. (1997) Selection of an adjuvant for vaccination with the malaria antigen, MSA-2. Vaccine 15, 1017ā1023.
Jacobsen, N. E., Fairbrother, W. J., Kensil, C. R., Lim, A., Wheeler, D. A., and Powell, M. F. (1996) Structure of the saponin adjuvant QS-21 and its base-catalyzed isomerization product by 1H and natural abundance 13C NMR spectroscopy. Carbohydr. Res. 280, 1ā14.
Cleland, J. L., Kensil, C. R., Lim, A., Jacobsen, N. E., Basa, L., Spellman, M., et al. (1996) Isomerization and formulation stability of the vaccine adjuvant QS-21. J. Pharm. Sci. 85, 22ā28.
Hoffman, S. L., Edelman, R., Bryan, J. P., Schneider, I., Davis, J., Sedegah, M., et al. (1994) Safety, immunogenicity and efficacy of a malaria sporozoite vaccine administered with monophosphoryl lipid A, cell wall skeleton of mycobacteria and squalene as adjuvant. Am. J. Trop. Med. Hyg. 51, 603ā612.
Onier, N., Hilpert, S., Reveneau, S., Arnould, L., Saint-Giorgio, V., Exbrayat, J. M., et al. (1999) Expression of inducible nitric oxide synthase in tumors in relation with their regression induced by lipid A in rats. Int. J. Cancer. 81, 755ā760.
Chang, M. F., White, J. L., Nail, S. L., and Hem, S. L. (1997) Role of the electrostatic attractive force in the adsorption of proteins by aluminum hydroxide adjuvant. PDAJ. Pharm. Sci. Technol. 51, 25ā29.
Al-Shakhshir, R., Regnier, F., White, J. L., and Hem, S. L. (1994) Effect of protein adsorption on the surface charge characteristic of aluminum-containing adjuvants. Vaccine 12, 472ā474.
Pellegrini, V., Fineschi, N., Matteucci, G., Marsili, I., Nencioni, L. Puddu, M., et al. (1993) Preparation and immunogenicity of an inactivated hepatitis A vaccine. Vaccine 11, 383ā387.
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Meraldi, V., Romero, J.F., Corradin, G. (2002). Peptide Vaccination. In: Doolan, D.L. (eds) Malaria Methods and Protocols. Methods in Molecular Medicineā¢, vol 72. Humana Press. https://doi.org/10.1385/1-59259-271-6:335
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DOI: https://doi.org/10.1385/1-59259-271-6:335
Publisher Name: Humana Press
Print ISBN: 978-0-89603-823-3
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