Abstract
The genetic identification of malaria parasites has become an important task for epidemiological studies and for laboratory-based studies (1–7). A large number of adapted field isolates have been cultured and widely circulated during the past years. However, only few of these have been clearly characterized by molecular means and in some instances it has been shown that parasites previously believed to be derived from different isolates show a similar to identical molecular makeup (8). Similarly, parasite cultures believed to be identical show significant differences on a genetic level (9). Such unreliability of sources of research might cause havoc to the studies and might contribute to discrepant findings.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Färnert, A., Rooth, I., Svensson, A., Snounou, G., and Björkman, A. (1999) Complexity of Plasmodium falciparum infections is consistent over time and protects against clinical disease in Tanzanian children. J. Infect. Dis. 179, 989–995.
Färnert, A., Snounou, G., Rooth, I., and Björkman, A. (1997) Daily dynamics of Plasmodium falciparum subpopulations in asymptomatic children in a holoendemic area. Am. J. Trop. Med. Hyg. 56, 538–547.
Brockman, A., Paul, R. E., Anderson, T. J., Hackford, I., Phaiphun, L., Looareesuwan, S., Nosten, F., and Day, K. P. (1999) Application of genetic markers to the identification of recrudescent Plasmodium falciparum infections on the northwestern border of Thailand. Am. J. Trop. Med. Hyg. 60, 14–21.
Ohrt, C., Mirabelli-Primdahl, L., Karnasuta, C., Chantakulkij, S., and Kain, K. C. (1997) Distinguishing Plasmodium falciparum treatment failures from reinfections by restrictions fragment length polymorphism and polymerase chain reaction genotyping. Am. J. Trop. Med. Hyg. 57, 430–437.
Viriyakosol, S., Siripoon, N., Petcharapirat, C., Petcharapirat, P., Jarra, W., Thaithong, S., et al. (1995) Genotyping of Plasmodium falciparum isolates by the polymerase chain reaction and potential uses in epidemiological studies. Bull. World Health Organ. 73, 85–95.
Felger, I., Tavul, L., Kabintik, S., Marshall, V., Genton, B., Alpers, M., and Beck, H. P. (1994) Plasmodium falciparum: extensive polymorphism in merozoite surface antigen 2 alleles in an area with endemic malaria in Papua New Guinea. Exp. Parasitol. 79, 106–116.
Irion, A., Felger, I., Abdulla, S., Smith, T., Mull, R., Tanner, M., et al. (1998) Distinction of recrudescences from new infections by PCR-RFLP analysis in a comparative trial of CGP 56 697 and chloroquine in Tanzanian children. Trop. Med. Int. Health. 3, 490–497.
Kahane, B., Sibilli, L., Scherf, A., Jaureguiberry, G., Langsley, G., Ozaki, L.S., et al. (1987) The polymorphic 11.1 locus of Plasmodium falciparum. Mol. Biochem. Parasitol. 26, 77–85.
Fandeur, T., Bonnefoy, S., and Mercereau-Puijalon, O. (1991) In vivo and in vitro derived Palo Alto lines of Plasmodium falciparum are genetically unrelated. Mol. Biochem. Parasitol. 47, 167–178.
Walliker, D., Quakyi, I. A., Wellems, T. E., McCutchan, T. F., Szarfman, A., et al. (1987) Genetic analysis of the human malaria parasite Plasmodium falciparum. Science 236, 1661–1666.
Sanderson, A., Walliker, D., and Molez, J. F. (1981) Enzyme typing of Plasmodium falciparum from African and some other Old World countries. Trans. R. Soc. Trop. Med. Hyg. 75, 263–26
Howard, J., Carlton, J. M., Walliker, D., and Jensen, J. B. (1996) Use of random amplified polymorphic DNA (RAPD) technique in inheritance studies of Plasmodium falciparum. J. Parasitol. 82, 941–9
Dolan, S. A., Herrfeldt, J.A., and Wellems, T. E. (1993) Restriction polymorphisms and fingerprint patterns from an interspersed repetitive element of Plasmodium falciparum DNA. Mol. Biochem. Parasitol. 61, 137–1
Jeffreys, A. J., Brookfield, J. F., and Semeonoff, R. (1985) Positive identification of an immigration test-case using human DNA fingerprints. Nature 6, 317, 818,819.
Su, X. Z., Carucci, D. J., and Wellems, T. E. (1998) Plasmodium falciparum: parasite typing by using a multicopy microsatellite marker, PfRRM. Exp. Parasitol. 89, 262–265.
Carcy, B., Bonnefoy, S., Schrevel, J., and Mercereau-Puijalon, O. (1995) Plasmodium falciparum: typing of malaria parasites based on polymorphism of a novel multigene family. Exp. Parasitol. 80, 463–472.
Church, G. M. and Gilbert, W. (1984) Genomic sequencing. Proc. Natl. Acad. Sci. USA 81, 1991–1995.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2002 Humana Press Inc.
About this protocol
Cite this protocol
Beck, HP. (2002). RFLP Analysis. In: Doolan, D.L. (eds) Malaria Methods and Protocols. Methods in Molecular Medicine™, vol 72. Humana Press. https://doi.org/10.1385/1-59259-271-6:205
Download citation
DOI: https://doi.org/10.1385/1-59259-271-6:205
Publisher Name: Humana Press
Print ISBN: 978-0-89603-823-3
Online ISBN: 978-1-59259-271-5
eBook Packages: Springer Protocols