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A Method for Generating Transgenic Frog Embryos

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Molecular Embryology

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 97))

Abstract

The early amphibian embryo has been widely used as a model organism for studying early vertebrate development. This chapter describes in detail a new and very efficient method for generating transgenic Xenopus embryos. At the end of the chapter, a new method for fertilizing in vitro matured oocytes is also introduced.

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Author information

Authors and Affiliations

  1. Wellcome, CRC Institute, Cambridge, UK

    Enrique Amaya & Kristen L. Kroll

Authors
  1. Enrique Amaya
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  2. Kristen L. Kroll
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Editor information

Editors and Affiliations

  1. Dental and Medical Schools of Guy’s, King’s, and St. Thomas’s Hospitals, King’s College, London, UK

    Paul T. Sharpe  & Ivor Mason  & 

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© 1999 Humana Press Inc.

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Amaya, E., Kroll, K.L. (1999). A Method for Generating Transgenic Frog Embryos. In: Sharpe, P.T., Mason, I. (eds) Molecular Embryology. Methods in Molecular Biology™, vol 97. Humana Press, Totowa, NJ. https://doi.org/10.1385/1-59259-270-8:393

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  • DOI: https://doi.org/10.1385/1-59259-270-8:393

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-387-0

  • Online ISBN: 978-1-59259-270-8

  • eBook Packages: Springer Protocols

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