Abstract
Cell lineage analyses trace the hierarchy of cell types derived from a progenitor population. Critical to these analyses is the ability to track reliably all or defined subsets of the clonal descendants of the progenitor population. This necessitates marking the cells with a heritable and cell autonomous marker. Transgenes encoding molecules that can be visualized directly in situ without compromising cell differentiation, such as the reporter, β-galactosidase encoded by lacZ, and chloramphenicol acyltransferase, encoded by the CAT gene are the most widely used.
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© 1999 Humana Press Inc.
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Trainor, P.A. et al. (1999). Application of lacZ Transgenic Mice to Cell Lineage Studies. In: Sharpe, P.T., Mason, I. (eds) Molecular Embryology. Methods in Molecular Biology™, vol 97. Humana Press, Totowa, NJ. https://doi.org/10.1385/1-59259-270-8:183
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DOI: https://doi.org/10.1385/1-59259-270-8:183
Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-59259-270-8
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