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UV-Visible Spectroscopy as a Tool to Study Flavoproteins

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Flavoprotein Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 131))

Abstract

The essential cofactor of flavoproteins is derived from riboflavin ( Fig. 1 ), which consists of the isoalloxazine ring system with a ribityl side chain attached to the central N-10 position in the pyrazine moiety.

Structure of riboflavin.

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References

  1. Massey, V. and Ghisla, S. (1983) in Biological Oxidations Vol. 34, pp. 114–139, Springer-Verlag, Mosbach, Germany.

    Google Scholar 

  2. Ghisla, S. and Massey, V. (1989) Mechanisms of flavoprotein-catalyzed reactions. Eur. J. Biochem. 181, 1–17.

    Article  PubMed  CAS  Google Scholar 

  3. Ghisla, S., Massey, V., Lhoste, J.-M., and Mayhew, S. G. (1974) fluorescence and optical characteristics of reduced flavines and flavoproteins. Biochemistry 13(3), 589–597.

    Article  PubMed  CAS  Google Scholar 

  4. Macheroux, P., Massey, V., Thiele, D. J., and Volokita, M. (1991) Expression of spinach glycolate oxidase in Saccharomyces cervisiae: Purification and characterization. Biochemistry 30, 4612–4619.

    Article  PubMed  CAS  Google Scholar 

  5. Müller, F., Brüstlein, M., Hemmerich, P., Massey, V., and Walker, W. H. (1972) Light-absorption studies on neutral flavin radicals. Eur. J. Biochem. 25(3), 573–580.

    Article  PubMed  Google Scholar 

  6. Massey, V. and Hemmerich, P. (1980) Active-site probes of flavoproteins. Biochem. Soc. Trans. 8, 246–257.

    PubMed  CAS  Google Scholar 

  7. Ghisla, S. and Massey, V. (1986) New flavins for old: artificial flavins as active site probes of flavoproteins. Biochem. J. 239, 1–12.

    PubMed  CAS  Google Scholar 

  8. Massey, V. and Palmer, G. H. (1966) On the existence of spectrally distinct classes of flavoprotein semiquinones. A new method for the quantitative production of flavoprotein semiquinones. Biochemistry 5, 3181–3189.

    Article  PubMed  CAS  Google Scholar 

  9. Mayhew, S. G. (1971) Studies on flavin binding in flavodoxins. Biochim. Biophys. Acta 235, 289–302.

    PubMed  CAS  Google Scholar 

  10. Macheroux, P., Hill, S., Austin, S., Eydmann, T., Jones, T., Kim, S.-O., Poole, R., and Dixon, R. (1998) Electron donation to the flavoprotein NifL, a redox-sensing transcriptional regulator. Biochem. J., 332, 413–419.

    PubMed  CAS  Google Scholar 

  11. Massey, V. and Hemmerich, P. (1977) A photochemical procedure for reduction of oxidation-reduction proteins employing deazariboflavin as catalyst. J. Biol. Chem. 252(16), 5612–5614.

    PubMed  CAS  Google Scholar 

  12. Massey, V. and Hemmerich, P. (1978) Photoreduction of flavoproteins and other biological compounds catalyzed by deazaflavins. Biochemistry 17(9), 9–17.

    Article  PubMed  CAS  Google Scholar 

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© 1999 Humana Press Inc., Totowa, NJ

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Macheroux, P. (1999). UV-Visible Spectroscopy as a Tool to Study Flavoproteins. In: Chapman, S.K., Reid, G.A. (eds) Flavoprotein Protocols. Methods in Molecular Biology, vol 131. Humana Press. https://doi.org/10.1385/1-59259-266-X:1

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  • DOI: https://doi.org/10.1385/1-59259-266-X:1

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-734-2

  • Online ISBN: 978-1-59259-266-1

  • eBook Packages: Springer Protocols

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