Abstract
The essential cofactor of flavoproteins is derived from riboflavin ( Fig. 1 ), which consists of the isoalloxazine ring system with a ribityl side chain attached to the central N-10 position in the pyrazine moiety.
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References
Massey, V. and Ghisla, S. (1983) in Biological Oxidations Vol. 34, pp. 114–139, Springer-Verlag, Mosbach, Germany.
Ghisla, S. and Massey, V. (1989) Mechanisms of flavoprotein-catalyzed reactions. Eur. J. Biochem. 181, 1–17.
Ghisla, S., Massey, V., Lhoste, J.-M., and Mayhew, S. G. (1974) fluorescence and optical characteristics of reduced flavines and flavoproteins. Biochemistry 13(3), 589–597.
Macheroux, P., Massey, V., Thiele, D. J., and Volokita, M. (1991) Expression of spinach glycolate oxidase in Saccharomyces cervisiae: Purification and characterization. Biochemistry 30, 4612–4619.
Müller, F., Brüstlein, M., Hemmerich, P., Massey, V., and Walker, W. H. (1972) Light-absorption studies on neutral flavin radicals. Eur. J. Biochem. 25(3), 573–580.
Massey, V. and Hemmerich, P. (1980) Active-site probes of flavoproteins. Biochem. Soc. Trans. 8, 246–257.
Ghisla, S. and Massey, V. (1986) New flavins for old: artificial flavins as active site probes of flavoproteins. Biochem. J. 239, 1–12.
Massey, V. and Palmer, G. H. (1966) On the existence of spectrally distinct classes of flavoprotein semiquinones. A new method for the quantitative production of flavoprotein semiquinones. Biochemistry 5, 3181–3189.
Mayhew, S. G. (1971) Studies on flavin binding in flavodoxins. Biochim. Biophys. Acta 235, 289–302.
Macheroux, P., Hill, S., Austin, S., Eydmann, T., Jones, T., Kim, S.-O., Poole, R., and Dixon, R. (1998) Electron donation to the flavoprotein NifL, a redox-sensing transcriptional regulator. Biochem. J., 332, 413–419.
Massey, V. and Hemmerich, P. (1977) A photochemical procedure for reduction of oxidation-reduction proteins employing deazariboflavin as catalyst. J. Biol. Chem. 252(16), 5612–5614.
Massey, V. and Hemmerich, P. (1978) Photoreduction of flavoproteins and other biological compounds catalyzed by deazaflavins. Biochemistry 17(9), 9–17.
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© 1999 Humana Press Inc., Totowa, NJ
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Macheroux, P. (1999). UV-Visible Spectroscopy as a Tool to Study Flavoproteins. In: Chapman, S.K., Reid, G.A. (eds) Flavoprotein Protocols. Methods in Molecular Biology, vol 131. Humana Press. https://doi.org/10.1385/1-59259-266-X:1
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DOI: https://doi.org/10.1385/1-59259-266-X:1
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