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DNA Topoisomerase Protocols pp 253–268Cite as

Immunoblot Analysis and Band Depletion Assays

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 94))

Abstract

Western blotting has been widely utilized to detect various polypeptides or polypeptide epitopes (e.g., posttranslational modifications) within cells (reviewed in 1-4). If the signals in samples being analyzed are compared to a suitable standard curve and appropriate internal standards are utilized to confirm equivalent loading of various samples, Western blotting appears to be a suitable method of quantitating polypeptides as well.

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Author information

Authors and Affiliations

  1. Division of Oncology Research. Mayo Clinic; Department of Pharmacology, Mayo Medical School, Rochester, MN, USA

    Scott H. Kaufmann & Phyllis A. Svingen

Authors
  1. Scott H. Kaufmann
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  2. Phyllis A. Svingen
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Editor information

Editors and Affiliations

  1. St. Jude Children’s Research Hospital, Memphis, TN, USA

    Mary-Ann Bjornsti

  2. Vanderbilt University School of Medicine, Nashville, TN, USA

    Neil Osheroff

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© 1999 Humana Press Inc.

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Kaufmann, S.H., Svingen, P.A. (1999). Immunoblot Analysis and Band Depletion Assays. In: Bjornsti, MA., Osheroff, N. (eds) DNA Topoisomerase Protocols. Methods in Molecular Biology, vol 94. Humana, Totowa, NJ. https://doi.org/10.1385/1-59259-259-7:253

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  • DOI: https://doi.org/10.1385/1-59259-259-7:253

  • Published:

  • Publisher Name: Humana, Totowa, NJ

  • Print ISBN: 978-0-89603-444-0

  • Online ISBN: 978-1-59259-259-3

  • eBook Packages: Springer Protocols

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