Abstract
This chapter will describe two approaches that utilize the polymerase chain reaction (PCR) to clone new members of previously known adhesion receptor families. These two approaches are useful to clone either alternatively spliced forms of previously known family members or novel molecules whose presence is suggested from protein data.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Languino, L. R. and Ruoslahti, E. (1992) An alternative form of the integrin β1 subunit with a variant cytoplasmic domain. J. Biol. Chem. 267, 7116–7120.
Suzuki, S., Huang, Z.-S., and Tanihara, H.. (1990) Cloning of an integrin β subunit exhibiting high homology with integrin β3 subunit. Proc. Natl. Acad. Sci. USA 87, 5354–5358.
Suzuki, S. and Naitoh, Y. (1990) Amino acid sequence of a novel integrin β4 subunit and primary expression of the mRNA in epithelial cells. EMBO J. 9, 757–763.
Sheppard, D., Rozzo, C., Starr, L., Quaranta, V., Erle, D. J., and Pytela, R. (1990) Complete amino acid sequence of a novel integrin β subunit (β6) identified in epithelial cells using the polymerase chain reaction. J. Biol. Chem. 265, 11,502–11,507.
Erle, D. J., Ruegg, C., Sheppard, D., and Pytela, R. (1991) Complete amino acid sequence of an integrin β subunit (β7) identified in leukocytes. J. Biol. Chem. 266, 11,009–11,016.
Suzuki, S., Sano, K., and Tanihara, H. (1991) Diversity of the cadherin family: evidence for eight new cadherins in nervous tissue. Cell Regul. 2, 261–270.
Tanihara, H., Sano, K., Heimark, R. L., St. John, T., and Suzuki, S. (1994) Cloning of five cadherins clarifies characteristic features of cadherin extracellular domain and provides further evidence for two structurally different types of cadherin. Cell Adhesion and Commun. 2, 15–26.
Donalies, M., Cramer, M., Ringwald, M., and Starzinski-Powitz, A. (1991) Expression of M-cadherin, a member of the cadherin multigene family, correlates with differentiation of skeletal muscle cells. Proc. Natl. Acad. Sci. USA 88, 8024–8028.
Tamura, R. N., Rozzo, C., Starr, L, Chambers, J., Reichardt, L. F., Cooper, H. M., and Quaranta, V. (1990) Epithelial integrin α6β4: complete primary structure of α6 and variant forms of β4. J. Cell Biol. 111, 1593–1604.
Ramaswamy, H. and Hemler, M. E. (1990) Cloning, primary structure and properties of a novel human integrin β subunit. EMBO J. 9, 1561–1568.
McLean, J. W., Vestal, D. J., Cheresh, D. A., and Bodary, S. C. (1990) cDNA sequence of the human integrin β5 subunit. J. Biol. Chem. 265, 17,126–17,131.
Pytela, R., Suzuki, S., Breuss, J., Erle, D. J., and Sheppard, D. (1994) Polymerase chain reaction cloning with degenerate primers: homology-based identification of adhesion molecules. Methods Enzymol. 245, 420–451.
Nakamura, Y., Wada, K., Wada, Y., Doi, H., Kanaya, S., Gojobori, T., and Ikemura, T. (1996) Codon usage tabulated from the international DNA sequence databases. Nucleic Acids Res. 24, 214–215.
Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory Press), Cold Spring Harbor, NY.
Wozney, J. M. (1990) Using purified protein to clone its gene. Methods Enzymol. 182, 738–751.
Haase, A. T., Retzel, E. F., and Staskus, K. A. (1990) Amplification and detection of lentiviral DNA inside cells. Proc. Natl. Acad. Sci. USA 87, 4971–4975.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1999 Humana Press Inc.
About this protocol
Cite this protocol
Woodard, A.S., Languino, L.R. (1999). Application of Polymerase Chain Reaction for the Discovery of New Adhesion Molecule Family Members. In: Dejana, E., Corada, M. (eds) Adhesion Protein Protocols. Methods in Molecular Biology, vol 96. Humana Press. https://doi.org/10.1385/1-59259-258-9:65
Download citation
DOI: https://doi.org/10.1385/1-59259-258-9:65
Publisher Name: Humana Press
Print ISBN: 978-0-89603-417-4
Online ISBN: 978-1-59259-258-6
eBook Packages: Springer Protocols