Purification Through Affinity Chromatography and Microsequencing

  • Cecilia Garlanda
Part of the Methods in Molecular Biology book series (MIMB, volume 96)


Production of monoclonal antibodies (MAbs) using adhesion-involved cell types as a source of antigens allows the identification of new molecules participating in the adhesion process (1, 2, 3). Immunoaffinity purification followed by protein microsequencing is one of the techniques used to characterize the new adhesion molecule identified and to determine at least in part its amino acidic sequence (4,5).


Elution Buffer Hamilton Syringe Cyanogen Bromide Immunoaffinity Column Coomassie Brilliant Blue Staining 
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Copyright information

© Humana Press Inc. 1999

Authors and Affiliations

  • Cecilia Garlanda
    • 1
  1. 1.Laboratory of Immunopharmacology, Department of Immunology and Cell BiologyMario Negri Institute of Pharmacological ResearchMilanoItaly

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