Integrin-Mediated Stimulation of Tyrosine Phosphorylation in Lymphoid Cells

  • Angela Gismondi
  • Angela Santoni
Part of the Methods in Molecular Biology book series (MIMB, volume 96)


Engagement of integrins by natural ligands or specific antibodies initiates biochemical signaling events within the cell, important for regulating different cell functions, such as migration, adhesion, proliferation, differentiation, apoptosis, and specific gene expression (1, 2, 3). Integrin-mediated cell adhesion has been shown to be accompanied by tyrosine phosphorylation of several proteins in a variety of cell types including lymphoid cells (4, 5, 6, 7).


Tyrosine Phosphorylated Protein Immunoaffinity Purification Washed Bead Protein Kinase Substrate Desired Time Period 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    Schwartz, M. A., Schaller, M. D., and Ginsberg, M. H. (1995) Integrins: emerging paradigms of signal transduction. Annu. Rev. Cell Dev. Biol. 11, 549–599.PubMedCrossRefGoogle Scholar
  2. 2.
    Clark, E. A., and Brugge, J. S. (1995) Integrins and signal transduction pathways: the road taken. Science 268, 233–239.PubMedCrossRefGoogle Scholar
  3. 3.
    Yamada, K. M. and Miyamoto, S. (1995) Integrin transmembrane signaling and cytoskeletal control. Curr. Opin. Cell Biol. 7, 681–689.PubMedCrossRefGoogle Scholar
  4. 4.
    Sato, T., Tachibana, K., Nojima, Y., D’Avirro, N., and Morimoto, C. (1995) Role of the VLA-4 molecule in T cell costimulation. Identification of the tyrosine phosphorylation pattern induced by the ligation of VLA-4. J. Immunol. 155, 2938–2947.PubMedGoogle Scholar
  5. 5.
    Tachibana, K., Sato, T., D’Avirro, N., and Morimoto, C. (1995) Direct association of pp125FAK with paxillin, the focal adhesion-targeting mechanism of pp125FAK. J. Exp. Med. 182, 1089–1100.PubMedCrossRefGoogle Scholar
  6. 6.
    Maguire, J. E., Danahey, K. M., Burkly, L. C., and van Seventer, G. A. (1995) T cell receptor-and β1 integrin-mediated signals synergize to induce tyrosine phosphorylation of focal adhesion kinase (pp125FAK) in human T cells. J. Exp. Med. 182, 2079–2090.PubMedCrossRefGoogle Scholar
  7. 7.
    Gismondi, A., Milella, M., Palmieri, G., Piccoli, M., Frati, L., and Santoni, A. (1995) Stimulation of protein tyrosine phosphorylation by interaction of NK cells with fibronectin via α4β1 and α5β1. J. Immunol. 154, 3128–3137.PubMedGoogle Scholar
  8. 8.
    Gismondi, A., Mainiero, F., Morrone, S., Palmieri, G., Piccoli, M., Frati, L., and Santoni, A. (1992) Triggering through CD16 or phorbol esters enhances adhesion of NK cells to laminin via very late antigen 6. J. Exp. Med. 176, 1251–1257.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 1999

Authors and Affiliations

  • Angela Gismondi
    • 1
  • Angela Santoni
    • 1
  1. 1.Department of Experimental MedicineUniversity “La Sapienza”RomeItaly

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