Leukocyte Transmigration Through Vascular Endothelium

An In Vitro Method
  • Paola Allavena
  • Aldo Del Maschio
Part of the Methods in Molecular Biology book series (MIMB, volume 96)


The endothelial lining of blood vessels forms an intact monolayer throughout the vascular system, and constitutes a critical barrier between the blood flow and the tissues beneath. Endothelial cells (EC) have a prominent role in the regulation of hemostasis, immunity, and inflammation; in particular, EC are active participants in regulating the traffic of cells and substances from blood to tissues and vice-versa (1,2).


Transwell Insert Lower Compartment Newborn Calf Serum Endothelial Cell Monolayer Adherent Leukocyte 
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Copyright information

© Humana Press Inc. 1999

Authors and Affiliations

  • Paola Allavena
    • 1
  • Aldo Del Maschio
    • 1
  1. 1.Laboratory for Cellular Immunology, Department of Immunology and Cell BiologyMario Negri Institute for Pharmacological ResearchMilanoItaly

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