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In situ Hybridization with RNA Probes

  • Georg Breier
Part of the Methods in Molecular Biology book series (MIMB, volume 96)

Abstract

Immunohistochemistry or immunofluorescence analyses are ideal techniques for localizing adhesive molecules in organs and tissues if reliable, well-characterized antibodies are available. In situ hybridization with RNA probes represents an alternative method for detecting mRNA encoding adhesive proteins (1). In comparison to immunological methods, in situ hybridization is more time-consuming and has a lower resolution at the cellular level. However, it is the method of choice if cloned cDNA probes for adhesive molecules have been isolated, but suitable antibodies are not available. Furthermore, in situ hybridization is often more sensitive than immunological methods and, thus, allows the detection of adhesive molecules that are expressed at low levels. Finally, the detection of mRNA allows their inambiguous determination of the site of synthesis of secreted molecules or of molecules whose extracellular domains can be proteolytically processed, and that, on release from their producer cells, may travel to distant locations in the tissue (2).

Keywords

Acetic Anhydride Fume Hood Liquid Scintillant Posthybridization Wash Sense Control Probe 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 1999

Authors and Affiliations

  • Georg Breier
    • 1
  1. 1.Institut-Abteilung-Molekuare-Zell-BiologieMax-Plank-Institut für Physiologische und Klinische Forschung-W. G. KerckhoffBad NauheimGermany

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