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Measurement of Intracellular Calcium Concentration Using Confocal Microscopy

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Calcium Signaling Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 114))

Abstract

Many cellular functions are tightly regulated by intracellular calcium concentrations ([Ca2+]i), and, therefore, the measurement of [Ca2+]i is of critical importance. To determine Ca2+-related cellular dynamics accurately, it is necessary to measure three-dimensionally resolved [Ca2+]i with sufficient temporal resolution to follow fast cellular responses that generate signal pulses and wave propagation. Several techniques have been developed to assay [Ca2+]i, but a revolution in the study of intracellular [Ca2+]i occurred when fluorescent dyes for Ca2+ were developed (1). Since then, fluorescent dyes and fluorescent microscopy have been used to observe resting and nonresting [Ca2+] in intact cells as well as in subcellular fractions.

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Author information

Authors and Affiliations

  1. Departments of Medicine and Pharmacology, Division of Cardiovascular Diseases, Mayo Clinic, Mayo Foundation, Rochester, MN

    Carmen Perez-Terzic

  2. Laboratoire de Physiopathologie Cardiovasculaire, INSERM, Montpellier, France

    Marisa Jaconi

  3. University of Kansas Medical Center, Kansas City, KS

    Lisa Stehno-Bittel

Authors
  1. Carmen Perez-Terzic
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  2. Marisa Jaconi
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  3. Lisa Stehno-Bittel
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Editor information

Editors and Affiliations

  1. University of Leicester, UK

    David G. Lambert

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© 1999 Humana Press Inc., Totowa, NJ

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Perez-Terzic, C., Jaconi, M., Stehno-Bittel, L. (1999). Measurement of Intracellular Calcium Concentration Using Confocal Microscopy. In: Lambert, D.G. (eds) Calcium Signaling Protocols. Methods in Molecular Biology™, vol 114. Humana Press. https://doi.org/10.1385/1-59259-250-3:75

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  • DOI: https://doi.org/10.1385/1-59259-250-3:75

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-597-3

  • Online ISBN: 978-1-59259-250-0

  • eBook Packages: Springer Protocols

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