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Integrin Gene Targeting

  • Protocol
Integrin Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 129))

Abstract

Gene ablation, also known as knockout, is a powerful method to analyze gene function in vivo. Many integrin genes have been disrupted already, confirming but also contradicting previous results (1). In addition, new functions have been revealed, significantly increasing our understanding of the biological roles of integrins. Knockout of the remaining integrins will similarly elucidate their function during development. In addition, knockins of subtle mutations (2,3) and conditional knockouts (4,5), resulting in tissue-specific or temporally restricted gene ablation, will allow us to assess structure-function relationships of integrins in vivo and to investigate the function of integrins in a specific cell type at a specific time point in development.

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Author information

Authors and Affiliations

  1. Max-Planck-Institute für Biochemie, Martinsried, Germany

    Jan F. Talts

  2. Department of Experimental Pathology, Lund University Hospital, Lund, Sweden

    Cord Brakebusch & Reinhard Fässler

Authors
  1. Jan F. Talts
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  2. Cord Brakebusch
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  3. Reinhard Fässler
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Editor information

Editors and Affiliations

  1. Berlex Biosciences, Richmond, California, USA

    Anthony Howlett

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© 1999 Humana Press Inc.

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Talts, J.F., Brakebusch, C., Fässler, R. (1999). Integrin Gene Targeting. In: Howlett, A. (eds) Integrin Protocols. Methods in Molecular Biology, vol 129. Humana Press. https://doi.org/10.1385/1-59259-249-X:153

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  • DOI: https://doi.org/10.1385/1-59259-249-X:153

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-569-0

  • Online ISBN: 978-1-59259-249-4

  • eBook Packages: Springer Protocols

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