Abstract
The molecular mechanisms underlying self-renewal of pluripotent embryonic stem (ES) cells is still poorly understood. Deciphering these mechanisms is of prime importance for at least two reasons: (1) ES cells derive from, and are closely related to, the pluripotent stem cells of the blastocyst, the founder cells of the whole embryo proper. Hence, they constitute a unique model for studying embryonic development at the time of implantation, when embryos are inacessible to experimental manipulation; and (2) Isolating and manipulating ES cells in species of economic or therapeutic interests is more difficult than in the mouse. It is likely that better defining their growth requirements will lead to major improvements in their culture conditions.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Savatier, P., Huang, S., Szekely, L., Wiman, K. G., and Samarut, J. (1994) Contrasting patterns of retinoblastoma protein expression in mouse embryonic stem cells and embryonic fibroblasts. Oncogene 9, 809–818.
Savatier, P., Lapillonne, H., van Grunsven, L. A., Rudkin, B. B., and Samarut, J. (1996) Withdrawal of differentiation inhibitory activity/leukemia inhibitory factor up-regulates D-type cyclins and cyclin-dependent kinase inhibitors in mouse embryonic stem cells. Oncogene 12, 309–322.
Weinberg, R. A. W. (1995) The retinoblastoma protein and cell cycle control. Cell 81, 323–330.
Bartek, J., Bartkova, J., and Lukas, J. (1997) The retinoblastoma protein pathway in cell cycle control and cancer. Exp. Cell Res. 237, 1–6.
Kerkhoff, E. and Rapp, U. R. (1998) Cell cycle targets of Ras/Raf signalling. Oncogene 17, 1457–1462.
Zhang, H. S., Gavin, M., Dahiya, A., Postigo, A. A., Ma, D., Luo, R. X., et al. (2000) Exit from G1 and S phase of the cell cycle is regulated by repressor complexes containing HDAC-Rb-hSWI/SNF and Rb-hSWI/SNF. Cell 101, 79–89.
Harbour, J. W., Luo, R. X., Dei Santi, A., Postigo, A. A., and Dean, D. C. (1999) Cdk phosphorylation triggers sequential intramolecular interactions that progressively block Rb functions as cells move through G1. Cell 98, 859–869.
Hansen, R. and Oren, M. (1997) p53: from inductive signal to cellular effect. Curr. Opin. Genet. Dev. 7, 46–51.
Aladjem, M. I., Spike, B. T., Rodewald, L. W., Hope, T. J., Klemm, M., Jaenisch, R., and Wahl, G. M. (1998) ES cells do not activate p53-dependent stress responses and undergo p53-independent apoptosis in response to DNA damage. Curr. Biol. 29, 145–155.
Prost, S., Bellamy, C. O., Clarke, A. R., Wyllie, A. H., and Harrison, D. J. (1998). p53-independent DNA repair and cell cycle arrest in embryonic stem cells. FEBS Lett. 425, 499–504.
Nichols, J., Zevnik, B., Anastassiadis, K., Niwa, H., Klewe-Nebenius, D., Chambers, I., et al. (1998) Formation of pluripotent stem cells in the mammalian embryo depend on the POU transcription factor Oct4. Cell 95, 379–391.
Smith, A. G. (1991) Culture and differentiation of embryonic stem cells. J. Tiss. Cult. Methods 13, 89–94.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2002 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Savatier, P., Lapillonne, H., Jirmanova, L., Vitelli, L., Samarut, J. (2002). Analysis of the Cell Cycle in Mouse Embryonic Stem Cells. In: Turksen, K. (eds) Embryonic Stem Cells. Methods in Molecular Biology™, vol 185. Springer, Totowa, NJ. https://doi.org/10.1385/1-59259-241-4:27
Download citation
DOI: https://doi.org/10.1385/1-59259-241-4:27
Publisher Name: Springer, Totowa, NJ
Print ISBN: 978-0-89603-881-3
Online ISBN: 978-1-59259-241-8
eBook Packages: Springer Protocols