Isolation of Differentially Expressed Genes Through Subtractive Suppression Hybridization

  • Oliver Dorian von Stein
Part of the Methods in Molecular Biology™ book series (MIMB, volume 175)


Despite the fact that the genetic blueprint in every cell is identical, an organism is composed of a multitude of different cell types. This cell type complexity or phenotypic differences are the result of differential expression of identical genes. For example, a normal cell expresses a different repertoire of genes than a tumor cell derived from the same cell type. Moreover, to identify those genes would provide an entry point for understanding the biologic processes responsible for these phenotypic differences. This in turn could lead to the development of novel therapeutic strategies against cancer.


Polymerase Chain Reaction Suppression Subtractive Hybridization Nest Polymerase Chain Reaction Bidistilled Water Saline Sodium Citrate 
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Copyright information

© Humana Press Inc., Totowa, NJ 2001

Authors and Affiliations

  • Oliver Dorian von Stein
    • 1
  1. 1.InDex Pharmaceuticals ABStockholmSweden

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