Direct Selection of cDNAs by Genomic Clones
During the last decade, major advances in genomic research have resulted in the development of a variety of tools and technologies for gene identification that were successfully applied to the construction of transcriptional maps and to the identification of genes responsible for genetic disorders. Once genomic DNA sequences become available, in silico gene identification will be the most widely used method. However, established techniques for gene identification will still be relevant, especially to identify specific genes, mapped to defined regions of the genome.
KeywordsSodium Dodecyl Sulfate Polymerase Chain Reaction Amplification Polymerase Chain Reaction Reaction Gene Identification Yeast Artificial Chromosome
- 3.Peterson A. S. (1998) Direct cDNA selection, in Genome Analysis: A Laboratory Manual (Birren, B., Green, E. D., Klapholz, S., Myers, R. M., and Roskams J., eds.), Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp. 159–171.Google Scholar
- 5.Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory, Plainview, NY.Google Scholar