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Direct Selection of cDNAs by Genomic Clones

  • Daniela Toniolo
Part of the Methods in Molecular Biology™ book series (MIMB, volume 175)

Abstract

During the last decade, major advances in genomic research have resulted in the development of a variety of tools and technologies for gene identification that were successfully applied to the construction of transcriptional maps and to the identification of genes responsible for genetic disorders. Once genomic DNA sequences become available, in silico gene identification will be the most widely used method. However, established techniques for gene identification will still be relevant, especially to identify specific genes, mapped to defined regions of the genome.

Keywords

Sodium Dodecyl Sulfate Polymerase Chain Reaction Amplification Polymerase Chain Reaction Reaction Gene Identification Yeast Artificial Chromosome 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2001

Authors and Affiliations

  • Daniela Toniolo
    • 1
  1. 1.Institute of Genetics, Biochemistry, and EvolutionCNRPaviaItaly

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