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Contiguation of Bacterial Clones

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Part of the Methods in Molecular Biology™ book series (MIMB,volume 175)

Abstract

For accurate assembly of a bacterial clone map (or contig), it is necessary to precisely detect the degree of overlap and map order of clones. One way is to generate fingerprints for all the clones in a given project. The overlap between clones is determined by the proportion of comigrating restriction fragments on either acrylamide or agarose gels, with mulitfold coverage of clones allowing rapid identification of artifacts such as rearranged or deleted clones.

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© 2001 Humana Press Inc., Totowa, NJ

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Humphray, S.J., Knaggs, S.J., Ragoussis, I. (2001). Contiguation of Bacterial Clones. In: Starkey, M.P., Elaswarapu, R. (eds) Genomics Protocols. Methods in Molecular Biology™, vol 175. Humana Press. https://doi.org/10.1385/1-59259-235-X:069

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  • DOI: https://doi.org/10.1385/1-59259-235-X:069

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-774-8

  • Online ISBN: 978-1-59259-235-7

  • eBook Packages: Springer Protocols

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