Construction of a Bacterial Artificial Chromosome Library

  • Sangdun Choi
  • Ung-Jin Kim
Part of the Methods in Molecular Biology™ book series (MIMB, volume 175)


Bacterial artificial chromosomes (BACs) represent a very useful cloning system for large DNA fragments and utilize the Escherichia coli F factor as their backbone. E. coli F factor allows strict copy number control of the clones so that they are stably maintained at one to two copies per cell (1). The stability of the cloned DNA during propagation in E. coli host is substantially higher in lower copy number vectors than in multi-copy counterparts.


Bacterial Artificial Chromosome Isolation Buffer Average Insert Size Partial Digestion Haploid Genomic Size 
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Copyright information

© Humana Press Inc., Totowa, NJ 2001

Authors and Affiliations

  • Sangdun Choi
    • 1
  • Ung-Jin Kim
    • 1
  1. 1.Division of BiologyCalifornia Institute of TechnologyPasadena

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