Construction of a Bacterial Artificial Chromosome Library
Bacterial artificial chromosomes (BACs) represent a very useful cloning system for large DNA fragments and utilize the Escherichia coli F factor as their backbone. E. coli F factor allows strict copy number control of the clones so that they are stably maintained at one to two copies per cell (1). The stability of the cloned DNA during propagation in E. coli host is substantially higher in lower copy number vectors than in multi-copy counterparts.
KeywordsBacterial Artificial Chromosome Isolation Buffer Average Insert Size Partial Digestion Haploid Genomic Size
- 1.Willetts, N. and Skurray, R. (1987) Structure and function of the F factor and mechanism of conjugation. J. Cell Mol. Biol. 2, 1110–1133.Google Scholar
- 6.Choi, S. and Wing, R. A. (2000) The construction of bacterial artificial chromosome (BAC) libraries from plants, in Plant Molecular Biology Manual (Gelvin, S. B. and Schilperoort, R. A., eds.), Kluwer Academic Publishers, Dordrecht, The Netherlands, pp. 45, 1–28.Google Scholar