Abstract
Adipocytes express the three major types of adrenoceptors (ARs), α1-, α2-, and β-ARs, each of them being further divided into three subtypes. With the exception of α1A-, α1B-, and α1D-ARs, which have not been described to directly influence cAMP production in fat cells, all the other AR subtypes are known to interact with the regulation of lipolytic activity through stimulatory (β1-, β2-, and β3-ARs) or inhibitory (α2A/D-, αa2C-, and putatively α2B-ARs) G-protein-coupled mechanisms (reviewed in ref. 1). This explains why, besides their direct quantification by radioligand-binding analyses, the assays on ARs described in this chapter will also include the measurement of one of the biological effects they regulate: lipolysis.
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Lafontan, M. and Berlan, M. (1993) Fat cell adrenergic receptors and the control of white and brown fat cell function. J. Lipid Res. 34, 1057–1091.
Mauriège, P., Galitzky, J., Berlan, M., and Lafontan, M. (1987) Heterogenous distribution of beta-and alpha2-adrenoceptor binding sites in human fat cells from various fat deposits: functional consequences. Eur. J. Clin. Invest. 17, 156–165.
Wieland, O. (1957) Eine enzymatiche methode zur bestimmung von glycerin. Biochem. Z. 239, 313–319.
Rosenthal, H. E. (1967) Graphical method for the determination and presentation of binding parameters in a complex system. Anal. Biochem. 20, 525–532.
Bradley, D. C. and Kaslow, H. R. (1989) Radiometric assays for glycerol, glucose, and glycogen. Anal. Biochem. 180, 11–16.
Kather, H., Schroder, F., and Simon, B. (1982) Microdetermination of glycerol using bacterial NADH-linked luciferase. Clin. Chim. Acta 120, 295–300.
Honnor, R. C., Dhillon, G. S., and Londos, C. (1985) cAMP dependent protein kinase and lipolysis in rat adipocytes. I. Cell preparation, manipulation and predictability in behavior. J. Biol. Chem. 260, 15,122–15,129.
Carpéné, C., Marti, L., Hudson, A., and Lafontan, M. (1995) Nonadrenergic imidazoline binding sites and amine oxydases activities in fat cells. Annals NY Acad. Sci. 763, 380–397.
Dunigan, C. D., Curran, P. K., and Fishman, P. M. (2000) Detection of β-adrenergic receptors by radiogland binding, in Methods in Molecular Biology, vol. 126: Adrenergic Receptor Protocols (Machida, C. A., ed), Humana, Totowa, NJ, pp. 329–343.
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Carpéné, C. (2001). Assays of Adrenergic Receptors. In: Ailhaud, G. (eds) Adipose Tissue Protocols. Methods in Molecular Biology™, vol 155. Springer, Totowa, NJ. https://doi.org/10.1385/1-59259-231-7:129
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DOI: https://doi.org/10.1385/1-59259-231-7:129
Publisher Name: Springer, Totowa, NJ
Print ISBN: 978-0-89603-747-2
Online ISBN: 978-1-59259-231-9
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