Abstract
The deposition of fat in adipose tissue (AT) is an important aspect of energy metabolism. AT fat originates either from ingested fatty acids (FAs) or from de novo synthesis. The synthesis of FAs is active in several tissues (liver, mammary gland, lung, intestine, brown and white ATs); however, in rodents, white AT is the major site of lipogenesis, and contributes to more than 50% of total FA biosynthesis. Lipogenesis from glucose is regulated by the nutritional state: It is decreased to a minimum in carbohydrate deficiency, and is considerably enhanced during carbohydrate availability.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Beaty, N. B. and Lane, M. D. (1983) The polymerization of acetyl-CoA carboxylase. J. Biol. Chem. 258, 13,051–13,055.
Martin, D. B. and Vagelos, P. R. (1962) The mechanism of tricarboxylic acid cycle regulation. J. Biol. Chem. 237, 1787–1792.
Allred, J. B. and Roehrig, K. L. (1980) Inhibition of rat liver acetylCoA carboxy-lase by chloride. J.Lipid Res. 21, 488–491.
Volpe, J. J. and Vagelos, P. R. (1976) Mechanisms and regulation of biosynthesis of saturated fatty acids. Physiol. Rev. 56, 339–417.
Halestrap, A. P. and Denton, R. M. (1973) Insulin and the regulation of adipose tissue acetyl coenzyme A carboxylase. Biochem. J. 132, 509–513.
Cottam, G. L. and Srere, P. A. (1969) The sulfhydryl groups of citrate cleavage enzyme. Arch. Biochem. Biophys. 130, 304–311.
Ochoa, S. (1955) Malic enzyme. Method Enzymol. 1, 739–753.
Glock, C. E. and McLean, P. (1953) Further studies on the properties and assay of glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase of rat liver. Biochem. J. 55, 400–408.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2001 Humana Press Inc.
About this protocol
Cite this protocol
Bazin, R., Ferré, P. (2001). Assays of Lipogenic Enzymes. In: Ailhaud, G. (eds) Adipose Tissue Protocols. Methods in Molecular Biology™, vol 155. Springer, Totowa, NJ. https://doi.org/10.1385/1-59259-231-7:121
Download citation
DOI: https://doi.org/10.1385/1-59259-231-7:121
Publisher Name: Springer, Totowa, NJ
Print ISBN: 978-0-89603-747-2
Online ISBN: 978-1-59259-231-9
eBook Packages: Springer Protocols