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Epstein-Barr Virus

The B95-8 Strain Map

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 174))

Abstract

This chapter summarizes the genes and mRNAs that have been mapped on to the B95-8 EBV genome. The complete sequence of this strain of Epstein-Barr Virus (EBV) was established (1,2) and data from many publications has been integrated into the map, which is an update of that published previously (3). The B95-8 strain grows well in laboratory culture and transforms human B lymphocytes efficiently, but B95-8 EBV has about 11.8 kb deleted relative to other strains of EBV. The sequence of that region has been determined in the Raji strain of EBV and a map has been published (4). Detailed literature citations for most of the features have been published (3). The map should be used in conjunction with the B95-8 EBV DNA sequence, which can be accessed in the European Molecular Biology Laboratory (EMBL; embl:ebv.seq) or Genbank databases. The feature tables shown in those database files have not yet been revised so the information shown in this map is considerably more up to date.

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References

  1. Baer, R., Bankier, A. T., Biggin, M. D., Deininger, P. L., Farrell, P. J., Gibson, T. J., et al. (1984) DNA sequence and expression of the B95–8 Epstein-Barr virus genome. Nature 310, 207–211.

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  2. Laux, G., Perricaudet, M., and Farrell, P. J. (1988) A spliced EBV gene expressed in immortalised lymphocytes is created by circularisation of the linear viral genome. EMBO J. 7, 769–774.

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  4. Parker, B. D., Bankier, A., Satchwell, S., Barrell, B., and Farrell, P. J. (1990) Sequence and transcription of Raji Epstein-Barr virus DNA spanning the B95-8 deletion. Virology 179, 339–346.

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© 2001 Humana Press Inc., Totowa, NJ

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Farrell, P.J. (2001). Epstein-Barr Virus. In: Wilson, J.B., May, G.H.W. (eds) Epstein-Barr Virus Protocols. Methods in Molecular Biology™, vol 174. Humana Press. https://doi.org/10.1385/1-59259-227-9:3

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  • DOI: https://doi.org/10.1385/1-59259-227-9:3

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-690-1

  • Online ISBN: 978-1-59259-227-2

  • eBook Packages: Springer Protocols

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