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Determination of Antigen and Fine Peptide Specificity of EBV-Specific CTLs

  • Protocol
Epstein-Barr Virus Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 174))

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Abstract

Currently, the antigen specificity of Epstein-Barr virus (EBV)-specific cytotoxic T cells (CTLs) can be determined using a set of recombinant vaccinia viruses expressing individual EBV proteins. These recombinant viruses are used to infect EBV-negative target cells, which are then tested for recognition by CTLs in standard cytotoxicity assay. Autologous fibroblasts are the most frequently used target cells for this type of experiment.

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References

  1. Murray, R. J., Kurilla, M. G., Griffin, H. M., Brooks, J. M., Mackett, M., Arrand, J. R., et al. (1990) Human cytotoxic T cell responses against Epstein-Barr virus nuclear antigens demonstrated using recombinant Vaccinia viruses. Proc. Natl. Acad. Sci. USA 87, 2906–2910.

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  2. Hirs, C. and Timasheff, S. (1977) Methods of peptide synthesis, in Enzyme Structure, vol. XLVII (Hirs, C. H. W. and Timesheff, S. N., eds.), Academic, New York, pp. 501–617.

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Author information

Authors and Affiliations

  1. Department of Tumour Biology, Karolinska Institutet, Stockholm, Sweden

    Victor Levitsky & Maria Masucci

  2. Microbiology and Tumor Biology Center (MTC), Karolinska Institutet, Stockholm, Sweden

    Teresa Frisan

Authors
  1. Victor Levitsky
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  2. Teresa Frisan
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  3. Maria Masucci
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Editor information

Editors and Affiliations

  1. I.B.L.S. Division of Molecular Genetics, University of Glasgow, UK

    Joanna B. Wilson

  2. I.B.L.S. Division of Biochemistry and Molecular Biology, University of Glasgow, UK

    Gerhard H. W. May

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© 2001 Humana Press Inc., Totowa, NJ

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Levitsky, V., Frisan, T., Masucci, M. (2001). Determination of Antigen and Fine Peptide Specificity of EBV-Specific CTLs. In: Wilson, J.B., May, G.H.W. (eds) Epstein-Barr Virus Protocols. Methods in Molecular Biology™, vol 174. Humana Press. https://doi.org/10.1385/1-59259-227-9:209

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  • DOI: https://doi.org/10.1385/1-59259-227-9:209

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-690-1

  • Online ISBN: 978-1-59259-227-2

  • eBook Packages: Springer Protocols

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