Abstract
Flow cytometry allows rapid multi-parameter analyses of individual cells. Cells are illuminated with incident laser light of a specific wavelength. Resultant light-scatter signals and fluorescence-emission signals from fluorochromes contained at the surface of the cell or within it are detected by an array of photomuliplier tubes. For example, fluorochrome-conjugated antibodies and DNA interchelating dyes can be used to acquire information on protein expression and DNA ploidy (via fluorescence signals) together with information on cell size and structure (via light-scatter signals).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Raff, M. C. (1992) Social controls on cell survival and cell death. Nature 356, 397–399.
Williams, G. T. and Smith, C. A. (1993) Molecular recognition of apoptosis: genetic controls on cell death. Cell 74, 777–779.
Hickman, J. A. (1992) Apoptosis induced by anticancer drugs. Cancer Metastasis Rev. 11, 121–139.
Wyllie, A. H. (1994) Apoptosis: death gets a break. Nature 369, 272, 273.
Anthoney, D. A., McIlwrath, A. J., Gallagher, W. M., Edlin, A. R. M., and Brown, R. (1996) Microsatellite instability, apoptosis and loss of p53 function in drug-resistant tumour cells. Cancer Res. 56, 1374–1381.
Ormerod, M. G., O’Neill, C. F., Robertson, D., and Harrap, K. (1994) Cisplatin induced apoptosis in a human ovarian carcinoma cell line without concomitant internucleosomal degradation of DNA. Exp. Cell Res. 211, 231–237.
Oberhammer, F., Wilson, J. W., Dive, C., Morris, I. D., Hickman, J. A., Wakeling, A. E., et al. (1993) Apoptotic death in epithelial cells: cleavage of DNA to 300 and/or 50 kb fragments prior to or in the absence of internucleosomal fragmentation. EMBO J. 12, 3679–3684.
Gorczyca, W., Gong, J., and Darzynkiewicz, Z. (1993) Detection of DNA strand breaks in individual apoptotic cells by the in situ terminal deoxynucleotidyl transferase and nick translation assays. Cancer Res. 53, 1945–1951.
Gorczyca, W., Bigman, K., Mitterman, A., Ahmed, T., Gong, J., Melamed, A. R., and Darzynkiewicz, Z. (1993) Induction of DNA strand breaks associated with apoptosis during the treatment of leukaemias. Leukaemia 7, 659–670.
Gregory, C. D., Dive, C., Henderson, S., Smith, C. A., Williams, G. T., Gordon, J., and Rickinson, A. B. (1991) Activation of Epstein Barr Virus latent genes protects human B cells from death by apoptosis. Nature 249, 612–614.
Ormerod, M. G. ed. (1990) Flow Cytometry: A Practical Approach. IRL Press, Oxford.
Chapman, R. S., Chresta, C. M., Herberg, A. A., Beere, H. M., Heer, S., Whetton, A. D., et al. (1995) Further characterisation of the in situ terminal deoxynucleotidyl transferase (TdT) assay for the flow cytometric analysis of apoptosis in drug resistant and drug sensitive leukaemic cells. Cytometry 20, 245–256.
Vermes, I., Haanen, C., and Reutelingsperger, C. (1995) A novel assay for apoptosis based upon flow cytometric detection of phosphatidylserine on the cell surface with use of FITC-labelled annexin V. Clin. Chem. 41, 91.
Dive, C., Gregory, C. D., Phipps, D. J., Evans, D. L., Milner, A. E., and Wyllie, A. H. (1992) Analysis and discrimination of necrosis and apoptosis (programmed cell death) by multi parameter flow cytometry. Biochim. Biophys. Acta 1133, 275–285.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2001 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Jones, N.A., Dive, C. (2001). Cell Sensitivity Assays. In: Wilson, J.B., May, G.H.W. (eds) Epstein-Barr Virus Protocols. Methods in Molecular Biology™, vol 174. Humana Press. https://doi.org/10.1385/1-59259-227-9:181
Download citation
DOI: https://doi.org/10.1385/1-59259-227-9:181
Publisher Name: Humana Press
Print ISBN: 978-0-89603-690-1
Online ISBN: 978-1-59259-227-2
eBook Packages: Springer Protocols