Abstract
Conventional gene knockout technology by homologous recombination can provide important information toward elucidating the function of some genes; however, the role of many genes cannot be investigated due to early embryonic lethality. Alternatively, the role of a particular gene in adult tissues may be masked by developmental abnormalities in any knockout animals generated. The implementation of site specific recombinases, such as the bacteriophage P1 LoxP/CRE system, enables the development of conditional knockouts that lack a particular gene only in a specific tissue or after a specific stage of development. This system can also be used to facilitate transgene activation/inactivation in vivo, deletions of large stretches of genomic DNA, chromosomal translocation and subtle alterations to genes and/or their regulatory sequences in vivo.
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© 2001 Humana Press Inc., Totowa, NJ
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Wilson, T.J., Kola, I. (2001). The LoxP/CRE System and Genome Modification. In: Tymms, M.J., Kola, I. (eds) Gene Knockout Protocols. Methods in Molecular Biology, vol 158. Humana Press. https://doi.org/10.1385/1-59259-220-1:83
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DOI: https://doi.org/10.1385/1-59259-220-1:83
Publisher Name: Humana Press
Print ISBN: 978-0-89603-572-0
Online ISBN: 978-1-59259-220-3
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