Abstract
Embryonic stem (ES) cells, the totipotent cells of early embryos established as permanent lines (1,2) retain their developmental capacity in vivo (3) as well as in vitro (see 4–6). The totipotent properties of ES cells are the basis of the gene-targeting technology to create mutant mice strains with inactivated genes by homologous recombination (7).
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Wobus, A.M., Guan, K., Pich, U. (2001). In Vitro Differentiation of Embryonic Stem Cells and Analysis of Cellular Phenotypes. In: Tymms, M.J., Kola, I. (eds) Gene Knockout Protocols. Methods in Molecular Biology, vol 158. Humana Press. https://doi.org/10.1385/1-59259-220-1:263
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DOI: https://doi.org/10.1385/1-59259-220-1:263
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