Abstract
The realization that much of the toxicity of bacterial endotoxin resulted from production of a macrophage-derived intermediate (1) led to the isolation and cloning of tumor necrosis factor (TNF), also known as cachectin (2). Since then, much evidence has accumulated to demonstrate that TNF is of prime importance in the pathogenesis of endotoxin-related tissue injury, and occupies a key proximal position in the cascade of mediators that are produced as a result of bacterial infection. These conclusions have been based in part on the accurate measurement of TNF in blood and other body fluids of humans and animals as a result of infection.
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References
Oettgen, H. F., Carswell, E. A., Kassel, R. L., Fiore, N., Williamson, B., Hoffmann, M. K., Haranaka, K., and and Old, L. J. (1980) Endotoxin-induced tumor necrosis factor. Recent Results Cancer Res. 75, 207–212.
Tracey, K. J., Beutler, B., Lowry, S. F., Merryweather, J., Wolpe, S., Milsark, I. W., Hariri, R. J., Fahey, T. J. I., Zentella, A., Albert, J. D., Shires, G. T., and Cerami, A. (1986) Shock and tissue injury induced by recombinant human cachectin. Science 234, 470–474.
Flick, D. A. and Gifford, G. E. (1984) Comparison of in vitro cell cytotoxic assays for tumor necrosis factor. J. Immunol. Methods 68, 167–174.
Angehrn, P., Banner, D., Braun, T., d’Arcy, A., Gehr, G., Gentz, R., Mackay, F., Schlaeger, E.-J., Schoenfeld, H., Loetscher, H., and Lesslauer, W. (1993) Two distinct tumor necrosis factor receptors in health and disease, in Tumor Necrosis Factor: Molecular and Cellular Biology and Clinical Relevance (Fiers, W. and Buurman, W. A., eds.), Karger, Basel, Switzerland, pp. 33–39.
Waage, A., Halstensen, A., and Espevik, T. (1987) Association between tumor necrosis factor in serum and fatal outcome in patients with meningococcal disease. Lancet i, 355–357.
Gray, P. W., Aggarwal, B. B., Benton, C. V., Bringman, T. S., Henzel, W. J., Jarrett, J. A., Leung, D. W., Moffat, B., Ng, P., Svedersky, L. P., Palladino, M. A., and Nedwin, G. E. (1984) Cloning and expression of cDNA for human lymphotoxin, a lymphokine with tumour necrosis activity. Nature 312, 721–724.
Sriskandan, S., Moyes, D., and Cohen, J. (1996) Detection of circulating bacterial superantigen and lymphotoxin-alpha in patients with streptococcal toxic-shock syndrome [letter]. Lancet 348, 1315–1316.
Wong, G. H., Elwell, J. H., Oberley, L. W., and Goeddel, D. V. (1989) Manganous superoxide dismutase is essential for cellular resistance to cytotoxicity of tumor necrosis factor. Cell 58, 923–931.
Pfister, H., Hennet, T., and Jungi, T. W. (1992) Lipopolysaccharide synergizes with tumour necrosis factor-alpha in cytotoxicity assays. Immunology 77, 473–476.
Branch, D. R., Shah, A., and Guilbert, L. J. (1991) A specific and reliable bioassay for the detection of femtomolar levels of human and murine tumor necrosis factors. J. Immunol. Methods 143, 251–261.
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Evans, T.J. (2000). Bioassay for Tumor Necrosis Factors-α and β. In: Evans, T.J. (eds) Septic Shock Methods and Protocols. Methods in Molecular Medicine™, vol 36. Humana Press. https://doi.org/10.1385/1-59259-216-3:83
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DOI: https://doi.org/10.1385/1-59259-216-3:83
Publisher Name: Humana Press
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