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Immunocytochemical Methods and Protocols pp 281–290Cite as

Assay for Phagocytosis

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 115))

Abstract

Phagocytic leukocytes (granulocytes, monocytes, and macrophages) provide a first line of host defense by their ingestion (phagocytosis) and killing of microorganisms. Leukocytes phagocytize particles through a complex series of events that include serum coating (opsonization), cellular recognition, particle adhesion to the cell surface, and internalization. Serum complement components are important for the particle adhesion to phagocytes, whereas IgG opsonization is necessary for particle internalization (reviewed in ref. 1).

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Author information

Authors and Affiliations

  1. Division of Hematology, Food and Drug Administration Center for Biologics Evaluation & Research, Bethesda, MD

    Liana Harvath

  2. Applied Information Technology Food & Drug Administration, Center for Biologics Evaluation & Research, Bethesda, MD

    Douglas A. Terle

Authors
  1. Liana Harvath
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  2. Douglas A. Terle
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Editor information

Editors and Affiliations

  1. Catholic University of America, Washington, DC

    Lorette C. Javois

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© 1999 Humana Press Inc., Totowa, NJ

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Harvath, L., Terle, D.A. (1999). Assay for Phagocytosis. In: Javois, L.C. (eds) Immunocytochemical Methods and Protocols. Methods in Molecular Biology™, vol 115. Humana Press. https://doi.org/10.1385/1-59259-213-9:281

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  • DOI: https://doi.org/10.1385/1-59259-213-9:281

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-813-4

  • Online ISBN: 978-1-59259-213-5

  • eBook Packages: Springer Protocols

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