Abstract
During the last century, before the advent of microtomes capable of producing thin, even sections, whole-mounts and thick sections were used to study the peripheral nervous system. Tissues were either viewed directly, if sufficiently thin, or further separated into layers by acid maceration (1). Initially, histochemical staining, particularly methylene blue (2), was applied to wholemounts to characterize neuronal cell types, distributions, and innervation of various organs. In more recent times precise innervations were traced through the use of histochemical techniques specific for neuronal components. Histofluorescence techniques were introduced by Falk (3) for the localization of nonadrenaline-containing nerves, and the discovery of neuropeptides led to the application of immunohistochemical techniques in the late 1970s and early 1980s (4,5). Most recently, the application of monoclonal antibody technology has opened a new era in research and diagnosis such that in situ normal and pathological tissue constituents may be detected in whole-mounts ranging from tissues to entire organisms.
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Meissner, G. (1857) Uber die Nerven der Darmwand. Z. Rationelle Med. 8, 364–366.
Erlich, P. (1886) Uber ide Methylenblau reaktion der lebenden Nervensubstang. Deutsch Med. Wschr. 12,49–52.
Falk, B. (1962) Observations on the possibilities of the cellular localization of monamines by a fluorescence method. Acta Physiol. Scand. 56, 19–25.
Costa, M., Patel, Y., Furness, J. B., and Arimura, A. (1977) Evidence that some intrinsic neurons of the intestine contain somatostatin. Neurosci. Lett. 6, 215–222.
Costa, M., Buffa, R., Furness, J. B., and Solcia, E. L. (1980) Immunohistochemical localization of polypeptides in peripheral antonomic nerves using whole-mount preparations. Histochemistry 65, 157–165.
Terada, M., Iwanaga, T., Takahasi-Iwanaga, H., Adachi, I., Arakawa, M., and Fujita, T. (1992) Calcitonin gene-related peptide (CGRP)-immunoreactive nerves in the tracheal epithelium of rats: an immunohistochemical study by means of whole-mount preparations. Arch. Histol. Cytol. 55, 219–233.
Liou, W. (1990) Whole-mount preparations of mouse lens epithelium for the fluorescent cytological study of actin. J. Microscopy 157, 239–245.
Tam, P. K. and Boyd, G. P. (1990) Origin, course, and endings of abnormal enteric nerve fibres in Hirschspring’ s disease defined by whole-mount immunohistochemistry. J. Ped. Surg. 25, 457–461.
Konig, N., Wilkie, M. B., and Lauder, J. M. (1988) Tyrosine hydroxylase and serotonin containing cells in embryonic rat rhombencephalon: a whole-mount immunocytochemical study. J. Neurosci. Res. 20, 212–223.
Javois, L. C., Wood, R. D., and Bode, H. R. (1986) Patterning of the head in hydra as visualized by a monoclonal antibody. I. Budding and regeneration. Dev. Biol. 117, 607–618.
Kuratani, S., Tanaka, S., Ishikawa, Y., and Zukeran, C. (1988) Early development of the hypoglossal nerve in the chick embryo as observed by whole-mount nerve staining method. Am. J. Anat. 182,155–168.
Plickert, G. and Kroiher, M. (1988) Proliferation kinetics and cell lineages can be studied in whole-mounts and macerates by means of BrdU/anti-BrdU technique. Develop. 103, 791–794.
Dent, J. A., Polson, A. G., and Klymkowsky, M. W. (1989) A whole-mount immunocytochemical analysis of the expression of the intermediate filament protein vimentin in Xenopus. Develop. 105, 61–74.
Gustafsson, M. K. (1991) Skin the tapeworms before you stain their nervous system: A new method for whole-mount immunocytochemistry. Parasitology Res. 77,509–516.
Tamaki, M., Iwanaga, T., Takeda, M., Adachi, I., Sato, S., and Fujita, T. (1992) Calcitonin gene-related peptide (CGRP)-immunoreactive nerve terminals in the whole-mount preparations of the dog urethra. Arch. Histol. Cytol. 55, 1–11.
Vielkind, U. and Swierenga, S. H. (1989) A simple fixation procedure for immunofluorescent detection of different cytoskeletal components within the same cell. Histochem. 91, 81–88.
Javois, L. C. and Tombe, V. K. (1990) Head activator does not qualitatively alter head morphology in regenerates ofHydra oligactis. Roux’s Arch. Dev. Biol. 199, 402–408.
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© 1999 Humana Press Inc., Totowa, NJ
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Javois, L.C. (1999). Fluorescent Labeling of Surface or Intracellular Antigens in Whole-Mounts. In: Javois, L.C. (eds) Immunocytochemical Methods and Protocols. Methods in Molecular Biology™, vol 115. Humana Press. https://doi.org/10.1385/1-59259-213-9:131
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DOI: https://doi.org/10.1385/1-59259-213-9:131
Publisher Name: Humana Press
Print ISBN: 978-0-89603-813-4
Online ISBN: 978-1-59259-213-5
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