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Direct Analysis of Chromosome Methylation

  • Déborah Bourc’his
  • Evani Viegas-Péquignot
Part of the Methods in Molecular Biology™ book series (MIMB, volume 181)

Abstract

DNA methylation is a possible candidate for a genomic imprinting marker in mammals. This epigenetic modification of DNA satisfies several essential criteria for the identification of the parental origin of individual alleles and larger portions of the genome: DNA methylation is stably propagated in somatic cells during cell division, it is reversible, it may inactivate the target sequence, and male and female gametes have different methylation patterns (reviewed in ref 1.).

Keywords

Glass Coverslip Hypotonic Buffer Hypotonic Treatment Label Nucleotide Germicidal Lamp 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Déborah Bourc’his
    • 1
  • Evani Viegas-Péquignot
    • 1
  1. 1.INSERM U383Hôpital Necker-Enfants MaladesParis CedexFrance

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