Abstract
Hyaluronan is a uniformly repetitive, linear glycosaminoglycan (GAG) composed ofdisaccharidesof glucuronic acid and N-acetylglucosamine: [-β(1,4)-GlcUA-β(1,3)- GlcNAc-]n. The polymer usually consists of 2,000–25,000 disaccharides, giving rise to molecular weights ranging from 106 to 107 dalton. Hyaluronan has unusual physical and biochemical properties, and fulfills several distinct physiological functions that contribute both to structural properties of tissues and to cell behavior during formation or remodeling of tissues (1–4). FIrst, hyaluronan contributes directly to tissue homeostasis and biomechanics due to its unique charge characteristics and biophysical properties. Second, interactions of hyaluronan with link proteins and proteoglycans are of fundamental importance to the structural integrity of extracellular and pericellular matrices. Third, interactions with cell-surface hyaluronan receptors mediate significant influences on cell behavior during morphogenesis, tissue remodeling, inflammation, and diseases such as cancer and atherosclerosis (1–4).
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References
Hascall, V. C. and Laurent, T. C. (1999) Hyaluronan: structure and physical properties. Glycoforum: Science of hyaluronan. http://www.glycoforum.gr.jp
Knudson, W. and Knudson, C. B. (1999) The hyaluronan receptor, CD44. Glycoforum: Science of hyaluronan. http://www.glycoforum.gr.jp
Sherman, L., Sleeman, J., Herrlich, P., and Ponta, H. (1994) Hyaluronate receptors: key players in growth, differentiation, migration and tumor progression. Curr. Opin. Cell Biol. 6, 726–733.
Toole, B. P. (2000) Hyaluronan, in Proteoglycans: Structure, Biology, and Molecular Interactions (Iozzo, R., ed.), Marcel Dekker, New York, NY, pp. 61–92.
Knudson, C. B. and Toole, B. P. (1985) Fluorescent morphological probe for hyaluronate. J. Cell Biol. 100, 1753–1758.
Delpech, B., Bertrand, P., Maingonnat, C., Girard, N., and Chauzy, C. (1995) Enzymelinked hyaluronectin: a unique reagent for hyaluronan assay and tissue location and for hyaluronidase activity detection. Anal. Biochem. 229, 35–41.
Fenderson, B. A., Stamenkovic, I. and Aruffo, A. (1993) Localization of hyaluronan in mouse embryos during implantation, gastrulation and organogenesis. Differentiation 54, 85–98.
Green, S. J., Tarone, G., and Underhill, C. B. (1988) Distribution of hyaluronate and hyaluronate receptors in the adult lung. J. Cell Sci. 90, 145–156.
Alho, A. M. and Underhill, C. B. (1989) The hyaluronate receptor is preferentially expressed on proliferating epithelial cells. J. Cell Biol. 108, 1557–1565.
Yu, Q. and Toole, B. P. (1997) Common pattern of CD44isoforms is expressed in morphogenetically active epithelia. Dev. Dyn. 208, 1–10.
Ripellino, J. A., Klinger, M. M., Margolis, R.U., and Margolis, R.K. (1985) The hyaluronic acid binding region as a specific probe for the localization of hyaluronic acid in tissue sections. J. Histochem. Cytochem. 33, 1060–1066.
Yang, B., Yang, B. L., Savani, R. C., and Turley, E. A. (1994) Identification of a common hyaluronan binding motif in the hyaluronan binding proteins RHAMM, CD44 and link protein. EMBO J. 13, 286–296.
Grammatikakis, N., Grammatikakis, A., Yoneda, M., Yu, Q., Banerjee, S. D., and Toole, B. P. (1995) A novel glycosaminoglycan-binding protein is the vertebrate homologue of the cell cycle control protein, Cdc37. J. Biol. Chem. 270, 16198–16205.
Yang, B., Yang, B. L., and Goetinck, P. F. (1995) Biotinylated hyaluronic acid as a probe for identifying hyaluronic acid-binding proteins. Anal. Biochem. 228, 299–306.
Melrose, J., Little, C. B., and Ghosh, P. (1998) Detection of aggregatable proteoglycan populations by affinity blotting using biotinylated hyaluronan. Anal. Biochem. 256, 149–157.
Yu, Q. and Toole, B. P. (1995) Biotinylated hyaluronan as aprobe for detection of binding proteins in cells and tissues. BioTechniques 19, 122–129.
English, N. M., Lesley, J. F., and Hyman, R. (1998) Site-specific de-N-glycosylation of CD44 can activate hyaluronan binding, and CD44 activation states show distinct threshold densities for hyaluronan binding. Cancer Res. 58, 3736–3742.
Kincade, P. W., Zheng, Z., Katoh, S., and Hanson, L. (1997) The importance of cellular environment to function of the CD44 matrix receptor. Curr. Opin. Cell Biol. 9, 635–642.
Tengblad, A. (1979) Affinity chromatography on immobilized hyaluronate and its application to the isolation of hyaluronate binding proteins from cartilage. Biochim. Biophys. Acta 578, 281–289.
Banerjee, S. D. and Toole, B. P. (1991) Monoclonal antibody to chick embryo hyaluronanbinding protein: changes in distribution of binding protein during early brain development. Dev. Biol. 146, 186–197.
Bitter, T. and Muir, H. (1962) A modified uronic acid carbazole reaction. Anal. Biochem. 4, 330–334.
Reissig, J. L., Strominger, J. L., and Leloir, L. R. (1955) A modified colorimetric method for the estimation of N-acetylamino sugars. J. Biol. Chem. 217, 959–966.
Zeng, C., Toole, B. P., Kiney, S. D., Kuo, J. W., and Stamenkovic, I. (1998) Inhibition of tumor growth in vivo by hyaluronan oligomers. Int. J. Cancer 77, 396–401.
Lesley, J., Kincade, P. W. and Hyman, R. (1993) Antibody-induced activation of the hyaluronan receptor function of CD44 requires multivalent binding by antibody. Eur. J. Immunol. 23, 1902–1909.
Underhill, C. B., Chi-Rosso, G., and Toole, B. P. (1983). Effects of detergent solubilization on the hyaluronate-binding protein from membranes of simian virus 40-transformed 3T3 cells. J. Biol. Chem. 258, 8086–8091.
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Toole, B.P., Yu, Q., Underhill, C.B. (2001). Hyaluronan and Hyaluronan-Binding Proteins Probes for Specific Detection. In: Iozzo, R.V. (eds) Proteoglycan Protocols. Methods in Molecular Biology™, vol 171. Humana Press. https://doi.org/10.1385/1-59259-209-0:479
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DOI: https://doi.org/10.1385/1-59259-209-0:479
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