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Cartilage and Smooth Muscle Cell Proteoglycans Detected by Affinity Blotting Using Biotinylated Hyaluronan

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Book cover Proteoglycan Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 171))

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Abstract

Chondrocytes and smooth muscle cells synthesise the large CS-rich proteoglycans aggrecan (1) and versican (2) respectively. Both proteoglycans are capable of interacting with hyaluronan to form molecular aggregates that have important tissue specific functional roles to play. Aggrecan is a major matrix component of cartilage, the aggrecan aggregates are physically entrapped within the collagenous extracellular matrix of this tissue, and it is the collective interplay between this collagenous network and the aggrecan aggregates that equips this tissue with its unique viscoelastic and hydrodynamic properties and the ability to provide an almost frictionless weight-bearing surface to articulating joints (3). Smooth muscle cell versican, in comparison, is a quantitatively minor component of blood vessels but nevertheless it may influence the physicochemical properties of the vessel wall (4). Although the exact functional role of versican within blood vessels has yet to be fully elucidated, it is known to accumulate in intimal lesions during atherosclerosis and is implicated in the entrapment of low-density lipoprotein in the arterial wall during atherogenesis. Such interactions are likely to influence both the viscoelasticity and permeability of the vessel wall.

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Author information

Authors and Affiliations

  1. Institute of Bone and Joint Research, Royal North Shore Hospital, St. Leonards, Sydney, Australia

    James Melrose

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  1. James Melrose
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Editors and Affiliations

  1. Department of Pathology, Anatomy, and Cell Biology, Thomas Jefferson University, Philadelphia, PA

    Renato V. Iozzo MD

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© 2001 Humana Press Inc., Totowa, NJ

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Melrose, J. (2001). Cartilage and Smooth Muscle Cell Proteoglycans Detected by Affinity Blotting Using Biotinylated Hyaluronan. In: Iozzo, R.V. (eds) Proteoglycan Protocols. Methods in Molecular Biology™, vol 171. Humana Press. https://doi.org/10.1385/1-59259-209-0:053

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  • DOI: https://doi.org/10.1385/1-59259-209-0:053

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-759-5

  • Online ISBN: 978-1-59259-209-8

  • eBook Packages: Springer Protocols

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